中文摘要：

目的：克隆小鼠IL-33基因全长编码区cDNA,并对其进行序列分析。方法：从BALB/c小鼠的脊髓组织中提取总RNA,逆转录为cDNA,用热启动PCR技术,扩增小鼠IL-33基因全长编码区cDNA,经双酶切后,克隆入pcDNA3.1（＋）载体中,构建真核表达载体pcDNA3.1-mIL-33,然后进行酶切鉴定与序列分析。结果：小鼠IL-33基因的PCR产物和重组载体经凝胶电泳和酶切鉴定、测序分析证实,其序列与GenBank中数据一致。小鼠IL-33基因的全长编码序列为801 bp,编码266个氨基酸。结论：小鼠IL-33基因成功的克隆并构建了其真核表达载体,为进一步进行IL-33的表达与功能研究奠定了基础。

英文摘要：

Objective：To clone and analyze the cDNA sequence encoding mouse interleukin-33 （IL-33） gene.Methods：Total RNA was prepared from the spinal cord of BALB/c mouse,cDNA fragment encoding IL-33 gene was amplified by RT-PCR using specific primers,and then was cloned into pcDNA3.1 vector.Recombinant eukaryotic expression vector pcDNA3,1-mlL-33 was constructed and was transformed into the host E.coli strain TOP10 for identification.Inserted IL-33 gene was sequenced.Results：Sequence analysis indicated that the cDNA of IL-33 had the whole length of 801 bp with a complete open reading frame,which encoded the product of 266 amino acids.The cDNA shared 100% homology with the sequence of mRNA for mouse IL-33 gene in GeneBank.Conclusion：The whole-length cDNA of mouse IL-33 was successfully cloned,which laid a foundation for further studying on its biological function.