中文摘要：

[目的]建立盐酸西布曲明、呋塞米和盐酸麻黄碱的快速高分离液相色谱-质谱联用(rapid resolution liquid chromatography-mass spectrometry,RRLC-MS)方法,检测7种减肥药中是否含有非法添加成分。[方法]色谱条件:Agilent SB-C18柱(2.1mm×50mm,5μm),乙腈(含0.1%甲酸)-0.1%甲酸水溶液为流动相进行梯度洗脱,流速0.2m L·min-1;质谱条件:电喷雾离子(electrospray ionization,ESI)源,以正负离子、多反应监测(multiple reaction monitoring,MRM)扫描模式检测减肥药中违禁成分。[结果]7种减肥药中均检测到盐酸西布曲明和盐酸麻黄碱,含量范围分别为0.39~4.29μg·g-1和1.63~8984.18μg·g-1,1种减肥药中检测到呋塞米,含量为8.71μg·g-1。[结论]本研究建立的快速高分离液相色谱-质谱联用法方便快捷、灵敏度好、准确度高,适用于减肥药中非法添加成分的定性定量检测。

英文摘要：

Objective]To establish a rapid resolution liquid chromatography-mass spectrometry(RRLC-MS) method for sibutramine hydrochloride, ephedrine hydrochloride and furosemide, which is the illegally added substances in diet pills. [Method]The chromatographic conditions: Agilent SB-C18 column(2.1mm×50mm, 5μm). The mobile phase consists of acetonitrile(containing 0.1% formic acid) and 0.1% formic acid solution, gradient elution with flow rate of 0.2mL·min-1; The MS conditions: electrospray ionization(ESI) source, with positive and negative ions, multiple reaction monitoring(MRM) mode to detect contents of prohibited substances in diet pills. [Result]Under the above conditions, all seven diet pills were detected with the sibutramine hydrochloride and ephedrine hydrochloride. The concentration range of the two substances was from 0.39 to 4.29μg·g-1 and from 1.63 to 8984.18μg·g-1 respectively. One drug contained furosemide, while the content was 8.71μg·g-1. [Conclusion]The method established was fast, convenient, had high sensitivity and high accuracy, which can detect prohibited substances quantitatively and qualitatively in diet pills.