中文摘要：

间充质的干细胞(MSC ) 广泛地在 allogeneic 干细胞移植被使用了。我们比较了 immunologic， MSC 的造血的特征源于整个人的脐的绳索(UC ) ，以及从 UC 的不同的节，包括羊膜的膜(AM ) ，沃顿的果冻(WJ ) ，和脐的容器(紫外) 。房间显型被流动 cytometry 检验。淋巴细胞转变测试和混合淋巴细胞反应被执行评估从 UC 导出的 MSC 的免疫调节的活动。cytokines 的 mRNA 表示被即时聚合酶链反应检测。造血的功能被文化 co MSC 学习， CD34 ⁺ 房间从绳索血孤立。我们的结果显示出包括 UC 与这四不同的节分开的那 MSC， WJ，紫外，并且 AM 有类似的生物特征。所有 MSC 有多系区别能力并且能区分进造骨细胞， adipocytes，和 chondrocytes。MSC 也以一种剂量依赖者方式禁止了 allogeneic T 房间的增长。cytokines 的相对 mRNA 表示被检验，并且结果显示出比 MSC 的有的更高的 interleukin-6 (IL6 ) ， IL11，干细胞因素，和 FLT3 表示从 UC 的特定的节导出的那 UCMSC。当 co 有教养时， CD34 ⁺ 房间有高繁殖效率， MSC 源于 UC， UCMSC 在之中是最有效的喂层的不同的节。我们的学习证明 MSC 能与支持特征的类似的 immunomodulation 和造血作用从整个 UC 或 UC 的特定的节被孤立。

英文摘要：

Mesenchymal stem cells （MSCs） have been widely used in allogeneic stem cell transplantation. We compared im- munologic and hematopoietic characteristics of MSCs derived from whole human umbilical cord （UC）, as well as from different sections of UCs, including the amniotic membrane （AM）, Wharton＇s jelly （WJ）, and umbilical vessel （UV）. Cell phenotypes were examined by flow cytometry. Lymphocyte transformation test and mixed lymphocyte reaction were performed to evaluate the immuno-modulatory activity of MSCs derived from UCs. The mRNA expression of cytokines was detected by real- time polymerase chain reaction. Hematopoietic function was studied by co-culturing MSCs with CD34＋ cells iso- lated from cord blood. Our results showed that MSCs separated from these four different sections including UC, W J, UV, and AM had similar biological characteristics. All of the MSCs had multi-lineage differentiation ability and were able to differentiate into osteoblasts, adipocytes, and chondrocytes. The MSCs also inhibited the proliferation of allogeneic T cells in a dose-dependent manner. The relative mRNA expression of cytokines was examined, and the results showed that UCMSCs had higher interleukin-6 （IL6）, ILll, stem cell factor, and FLT3 expression than MSCs derived from specific sections of UCs. CD34＋ cells had high propagation efficiencies when co-cultured with MSCs derived from different sections of UCs, among which UCMSCs are the most efficient feeding layer. Our study demonstrated that MSCs could be isolated from whole UC or specific sections of UC with similar immuno- modulation and hematopoiesis supporting characteristics.