为了确定水酶法提取板栗壳棕色素的工艺条件,以板栗壳为原料,通过单因素试验和正交试验,对其工艺条件进行筛选。结果表明,水酶法提取板栗壳棕色素的最适工艺条件为:复合酶总酶量2.0%,纤维素酶、中性蛋白酶、果胶酶质量比为2:3:5,酶处理pH值5.0,温度50℃,时间2.0h,料液比1:120。
In order to obtain the optimal extraction process conditions of brown pigment from chestnut shell by enzymatic hydrolysis method, taking chestnut shell as materials, the process conditions were screened by several single factor experiments and a orthogonal experiment. The results showed that the optimal extraction process conditions were 2.0% composite enzymes, the mass ratio 2 : 3 : 5 of cellulose, neutral protease and pectinase, reaction pH value 5.0, temperature 50 ℃, duration 2.0 h, and solid-liquid ratio 1 : 120.