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设施菜田不同碳氮管理对反硝化菌结构和功能的影响
  • ISSN号:0253-2654
  • 期刊名称:《微生物学通报》
  • 时间:0
  • 分类:S451.241[农业科学—植物保护]
  • 作者机构:[1]安徽农业大学农学院,安徽合肥230036, [2]中国农业大学资源与环境学院,北京100193
  • 相关基金:国家自然科学基金项目(No.41230856,41301258); 安徽省自然科学基金项目(No.1408085QC68)
中文摘要:

【目的】通过6年长期定位试验,比较设施菜田不同碳氮管理下反硝化菌结构和功能的差异。【方法】采用末端限制性片段多态性(T-RFLP)和变性梯度凝胶电泳(DGGE)方法分别分析nir K/nir S和nos Z型反硝化菌群结构特征,利用自动连续在线培养监测体系(Robot系统)测定分析NO/(NO3-+NO2-)和N2O/(N2O+N2)产物比,并通过乙炔抑制法测定反硝化酶活性。【结果】传统施肥处理(CN)显著改变了nir K和nos Z型反硝化菌的结构,增加了NO/(NO3-+NO2-)和N2O/(N2O+N2)产物比。nir S型菌受碳氮管理影响较小。减氮(RN)和添加秸秆处理(RN+S)的nir K和nos Z型反硝化菌结构与CN处理的差异性显著,且会显著降低NO/(NO3-+NO2-)和N2O/(N2O+N2)产物比;与CN和RN相比,RN+S显著增加反硝化酶活性。【结论】设施菜田长期传统施肥措施改变了反硝化菌的结构和功能,增加土壤自身的NO产生能力并减弱了N2O还原N2的能力。减氮和添加秸秆管理能形成自身的反硝化菌群结构,并降低NO和N2O排放风险;秸秆的添加会促进反硝化潜在速率,降低菜田NO3-淋洗风险。

英文摘要:

[Objective] Based on a 6-years greenhouse cropping field, the objective of the present study was to find out the differences of the structure and function of denitrifier community under different carbon and nitrogen managements. [Methods] We used terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE) methods to analyze the structure of microbial communities containing nirK/nirS and nosZ, respectively, a robotized incubation system to measure NO/(NO3-+NO2-) and N2O/(N2O+N2) product ratio and an acetylene inhibition method to determine denitrification enzyme activity. [Results] Conventional N management (CN) significantly changed the structure of denitrifier communities containing nirK and nosZ and increased the NO/ (NO3 +NO2-) and N2O/(N2O+N2) product ratio, nirS-denitrifiers were less affected by carbon and nitrogen managements. Reduced N (RN) and reduced N plus straw (RN+S) management had significant changed the denitrifier communities containing nirK and nosZ and reduced NO/(NO3-+NO2-) and N2O/(N2O+N2) product ratio compared with CN treatment. In contrast to CN and RN treatments, RN+S significantly increased denitrification enzyme activity. [Conclusion] Conventional N management significantly changed the structure and function of denitrifier community, and promoted the formation of NO and N20. RN and RN+S managements could form new denitrifier community structure addition, straw application promoted potential leaching in greenhouses. and reduced the risk of NO and N2O emissions. In denitrification rates and reduced the risk of NO3-leaching in greenhouses.

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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编:赫荣乔
  • 地址:北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编:100101
  • 邮箱:xuj@sun.im.ac.cn
  • 电话:010-64807511
  • 国际标准刊号:ISSN:0253-2654
  • 国内统一刊号:ISSN:11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:29487