To identify human thermal comfort in naturally ventilated buildings,the research based on both subjective and objective data was carried out in Chongqing,P. R. China. The characteristics of subjects’ clothing regulation function,changes of actual mean thermal comfort vote (AMV) varying with time and acceptable operative temperature in natural conditions were analyzed. In addition,the indicator actual mean vote-actual percentage dissatisfied (AMV-APD) was used to study the actual dissatisfaction with thermal environment. The results indicate that regulative ability by changing clothing under natural ventilated conditions is very significant but limited simultaneously,about 1.7 ℃ per 0.1 clo. Under naturally ventilated conditions,people may have an acceptable operative temperature of 16-28 ℃. Based on the AMV-APD,the actual minimum percentage dissatisfied can reach 4% at AMV of -0.36.