欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
采用反相高效液相色谱（RP-HPLC-UV）和电喷雾飞行时间质谱（ESI-TOF/MS）、大气压化学电离质谱（APCI-MS）,分析烟叶中的茄尼醇。使用反相C4色谱柱,以V（甲醇）∶V（水）=9∶1为流动相,茄尼醇和烟草中的其它成分分离良好;茄尼醇在ESI正离子全范围扫描中主要形成[M-H2O＋H]＋和[M＋NH4]＋离子,只有微弱的[M＋H]＋离子,同时会产生一些碎片离子;而在APCI正离子全范围扫描中主要形成[M-H2O＋H]＋离子,检测不到[M＋H]＋离子,碎片离子也很少;通过对茄尼醇的ESI-TOF/MS和APCI-MS的质谱分析特征比较可以发现,茄尼醇在ESI源分析中的信号强度远远小于在APCI源分析中的信号强度,说明APCI源更适于茄尼醇的定量分析。
英文摘要：
A new method based on high-performance liquid chromatography （HPLC）, positive ion electrospray-time of flight mass spectrometry （ESI-TOF/MS） and atmospheric pressure chemical ionization mass spectrometry （APCI-MS） has been developed for the characterization of solanesol in extract of tobacco leaves. Solanesol and other compounds in tobacco leaf extract were separated on an Hypersil C4（4.6 nan x 150 nan） column with methanol and water （90： 10, V/V） as mobile phase, flow-rate is 0.8 mL/min, detection wavelength of UV is 211 nm. Within the scanned mass range, two major fragment ions were observed at m/z 613.5705 （[M- H2O＋ H]^＋ ） and 648.6072 （[M＋ NH4]^＋ ）, while small percentages （2% - 6% of the base peak） of the protonate [M ＋ H]^＋ ion （observed at m/z 631.5811） were also detected sometimes for the ESI＋ analysis. In the APCI^＋ mode, abundant stable [M - H2O ＋ H]^＋ ion （ m/z at 613.5） was also observed, but can not observe other ion and fragmentation. By comparing APCI-MS and ESI-TOF/ MS for the analysis of solanesol in extract of tobacco leaf, it is found that the APCI mode is more sensitive than ESI mode, so APCI mode is more suitable for solanesol quantitative analysis.
同期刊论文项目
同项目期刊论文
期刊信息
位置：