中文摘要：

丙烯酰胺是-种重要的酰胺类化合物,其聚合产物-聚丙烯酰胺被广泛应用于三次采油、水处理、造纸、冶金等领域.以腈水合酶为核心的生物催化法,尤其是游离细胞催化法,具有转化效率高、产物纯度高以及环境友好等优点,是目前丙烯酰胺生产的主要方法;而腈水合酶及其生产菌株的性能在丙烯酰胺生产工艺中起着至关重要的作用.分别从细胞层次和酶层次对常用的生产菌株性能改造策略进行了简介,并重点阐述了目前用于腈水合酶生产菌株改造的基因重组策略.最后介绍了腈水合酶和产酶细胞的协同改造策略及改造效果.其中,通过对产腈水合酶红球菌R. ruber TH在酶和细胞层面的协同改造,重组游离细胞TH8实现了工业规模约500g/L高浓丙烯酰胺的三批次水合制备.

英文摘要：

Acrylamide is one kind of important amide compounds. Polyacrylamide, the polymerized product of acrylamide, has been widely used in enhanced oil recovery, water treatment, papermaking industry, metallurgy and so on. Because of the advantages such as high productivity, high product purity and environmental-friendly, biocatalysis approach using the free resting cells harboring nitrile hydratase as the biocatalysts is the main method to produce acrylamide so far. As a result, the performances of nitrile hydratase and its producing strains play a vital role in the whole process. In this paper, we summarized the strategies commonly used to evolve the engineered strains from both the cell level and the enzyme level; and especially highlighted the superior engineered strains harboring nitrile hydratase for acrylamide production. Finally, we introduced a synergistic evolution strategy for the cells of Rhodococcus and in-cell nitrile hydratases. With this method, we obtained the engineered strain R. ruber TH8. During the industrial scale bioproduction of acrylamide through free-cell catalysis of TH8, about 500 g/L acrylamide were obtained and the recombinant cells could be reused for three batches.