中文摘要：

采用药膜法测试了脱氧鬼臼毒素对美洲大蠊Periplaneta ometicana初孵若虫的触杀活性，并测定了其对成虫中枢神经系统乙酰胆碱酯酶（AChE）和腺苷三磷酸酶（ATPase）离体活性的影响。结果表明：脱氧鬼臼毒素对美洲大蠊初孵若虫具有较强的毒杀活性，在接触时间24、48、72和96h的LC50分别为26．26、4．68、1．51和0．62μg／cm^2；其对AChE没有明显影响；对Na^＋ -K^＋ -ATPase有明显抑制作用，并存在浓度．效应关系，IC50为44．9μmol／L；对ca^2＋ -Mg^2＋ -ATPase表现出低剂量激活，高剂量抑制的现象。结果提示美洲大蠊的AChE不是脱氧鬼臼毒素靶标。而ATPase可能是脱氧鬼臼毒素的重要靶标之一。

英文摘要：

The contact toxicity of deoxypodophyllotoxin to newly-hatched Periplaneta americana larvae was tested with the method of drug film, and its effects on the in vitro activities of AChE and ATPase in central nerve system of adult P. americana were investigated. The results indicated that deoxypodophyllotoxin exhibited high toxicity against P. americana larvae, and its LC50 values were 26.26, 4.68, 1.51, and 0.62 μg/cm^2 at 24, 48, 72, and 96 h of exposure time, respectively. Though deoxypodophyllotoxin did not affect the activity of ACHE, it inhibited Na^＋ -K^＋ -ATPase in dose-dependent manner at the range of 5 - 625μmol/L concentration and the IC50 was 44.9 μmol/L. Deoxypodophyllotoxin showed activating effect at lower concentration while inhibitory effect at higher concentration on Ca^2＋ -Mg^2＋ -ATPase. The results suggest that AChE is not target of deoxypodophyllotoxin, while ATPase may be one of important targets of deoxypodophyllotoxin.