中文摘要：

背景：IKVAV肽序列是层粘连蛋白中促进神经细胞的黏附、生长及分化的活性区域，可通过自组装成凝胶形成一种新型的组织工程支架材料。 目的：设计合成含IKVAV肽（C16H31O-AAAAGGGEIKVAV），在PBS溶液触发下，观察其自组装成三维多孔凝胶。 设计、时间及地点：单一样本实验，于2004—09／2005—01在协和骨科实验室完成。 材料：固相法合成C16H31O—AAAA GGGEIKVAV。 方法：高效液相色谱仪测所得寡肽纯度，质谱仪测肽的相对分子质量，配制成1％肽．pH值调整为9．5，加入等体积的PBS溶液，透射电镜观察白组装凝胶超微结构。 主要观察指标：实验所得寡肽的纯度、相对分子质量，寡肽自组装后的大体观察及透射电镜观察其内部结构。 结果：多肽纯度为95％，其相对分子质量为1351．6，与寡肽相对分子质量的理论值一致。1％肽溶液在PBS溶液触发下数秒钟形成凝胶，透射电镜示自组装的凝胶为交织成网状的纤维结构，直径3-6nm，长度100-1500nm。 结论：实验合成了含IKVAV肽，在磷酸盐缓冲液中可自组装成多孔凝胶结构。

英文摘要：

BACKGROUND： IKVAV-containing peptide sequence is an active region that promotes the adhesion, growth, and differentiation of neural cells in the laminin. It can be fabricated into a novel tissue-engineered scaffold material by self-assembly into hydrogel. OBJECTIVE： This study was designed to synthesize IKVAV-containing （C16H31O-AAAA GGGEIKVAV） peptide. The peptide was observed self-assembly into three-dimensional and porous hydrogel after triggered with phosphate buffered saline （PBS）. DESIGN, TIME AND SETTING： Single-sample experiment, performed in the Laboratory of Department of Orthopedics, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology between September 2004 and January 2005. MATERIALS： IKVAV-containing peptide was synthesized by solid phase method. METHODS： Peptide purity and relative molecular mass were detected by high performance liquid chromatogram and mass-spectrometry, respectively. 1% peptide, whose pH value was equal to 9.5, was self-assembled into hydrogel with the addition of PBS. The ultramicrostructure of the hydrogel was observed through the use of transmission electron microscope （TEM）. MAIN OUTCOME MEASURES： Peptide purity and relative molecular mass were detected. Moreover, gross observation of the peptide after self-assembly and TEM observation of peptide ultramicrostructure were performed. RESULTS： Peptide relative molecular mass was 1351.6, which was in accordance with its theoretical value. Peptide purity was 95%. After triggered with PBS, 1% peptide was self-supported into hydrogel in a few seconds. TEM results showed that self-assembled hydrogel consisted of the interconnected nanofibers which varied from 3 to 6 nm in diameter and 100 to 1 500 nm m in length. CONCLUSION： The IKVAV-containing peptide was synthesized and self-organized successfully into porous hydrogel, which was triggered with PBS solution.