中文摘要：

目的：观察化疗诱导的卵巢早衰大鼠接受补肾阴法和补肾阳法干预后,其外周血肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）水平及卵巢颗粒细胞凋亡情况。方法：48只雌性SD大鼠随机分成对照（control）组、环磷酰胺（CTX）组、环磷酰胺＋补肾阴（CTX＋kidney yin）组、环磷酰胺＋补肾阳（CTX＋kidney yang）组。ELISA法检测各组大鼠外周血TNF-α、IFN-γ水平;末端脱氧核糖核苷酸转移酶介导的缺口末端标记（TUNEL）法检测卵巢颗粒细胞凋亡;Western blot法检测卵巢Bax、Bcl-2蛋白的表达。结果：CTX组大鼠外周血TNF-α、IFN-γ表达水平上升,卵巢颗粒细胞凋亡明显,卵巢中Bax蛋白明显增多,而Bcl-2蛋白明显减少。经本实验中补肾方药治疗后,外周血TNF-α、IFN-γ水平下降,颗粒细胞凋亡程度减轻,Bax蛋白减少,Bcl-2蛋白增多,部分指标变化以CTX＋kidney yin组较为明显。结论：补肾阴及补肾阳法可通过调控颗粒细胞凋亡相关的Bax、Bcl-2蛋白及外周血TNF-α、IFN-γ的水平,抑制卵巢颗粒细胞凋亡,恢复卵巢功能、增强储备能力。

英文摘要：

AIM：To treat the chemotherapy-induced premature ovarian failure（POF）rats with the methods of tonifying kidney yin and yang by observing the indicator changes in apoptosis of ovarian granulosa cells and related cytokines before and after medication. METHODS：SD rats（n = 36）were chronically given intraperitoneal injection of cyclophosphamide（CTX）for 15 d in order to establish the animal model of POF. The rats were randomly divided into control group,CTX group,CTX ＋ kidney yin group and CTX ＋ kidney yang group. The levels of tumor necrosis factor alpha（TNF-α）and interferon gamma（IFN-γ）in the peripheral blood were detected by ELISA. The apoptosis of ovarian granulosa cells was observed by TUNEL method. The levels of apoptosis related proteins were determined by Western blot. RESULTS：After modeling,the levels of TNF-α and IFN-γ in the peripheral blood were significantly increased. The protein expression of Bax was up-regulated and Bcl-2 was down-regulated. The apoptosis of ovarian granulosa cells was significantly induced with a deep staining. After treatment with tonifying kidney yin and yang,the levels of TNF-α and IFN-γ in the peripheral blood were decreased,the protein expression of Bax was down-regulated and Bcl-2 was up-regulated,and the apoptosis of ovarian granulosa cells was reduced. CONCLUSION：The reserve function of the ovarian can be recovered by prescriptions of tonifying the kidney yin and yang via regulating the expression of TNF-α,IFN-γ,Bax and Bcl-2,and inhibiting the apoptosis of ovarian granulosa cells.