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DNA methylation signature of intergenic region involves in nucleosome remodeler DDM1-mediated repression of aberrant gene transcriptional read-through
  • ISSN号:1673-8527
  • 期刊名称:《遗传学报:英文版》
  • 时间:0
  • 分类:Q78[生物学—分子生物学] Q75[生物学—分子生物学]
  • 作者机构:State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
  • 相关基金:We thank the ABRC for providing the Arabidopsis T-DNA inser- tion mutant seeds. This work was supported by Foundation for Innovative Research Group of the National Natural Science Foun- dation of China (No. 31121002).
中文摘要:

The strict and efficient transcription termination is required to prevent production of aberrant readthrough transcripts that may cause transcriptional interference at neighboring genes.However,the exact regulatory mechanism remains poorly understood.Through a genetic screening of a LUGFERASE(LUC) reporter system,we found that Arabidopsis nucleosome remodeler DECREASE IN DNA METHYLATION1(DDM1) is a key component of this regulatory machinery and plays an important role in transcription termination,thus limiting transcriptional read-through(TRT).By whole-genome strandspecific RNA sequencing,we identified and confirmed 43 endogenous TRTs between genes,transposable elements(TEs),or genes and TEs in the ddml-10 mutant,which mainly occurred at heterochromatin regions.The DNA metliylation analysis of these TRT regions revealed that TRT occurred frequently at the intergenic regions with a higher methylation level in wild type comparing to the regions where TRT did not occur.Our results suggest that the level of intergenic DNA methylation may involve in preventing aberrant gene TRT or producing new gene during evolution.

英文摘要:

The strict and efficient transcription termination is required to prevent production of aberrant read- through transcripts that may cause transcriptional interference at neighboring genes. However, the exact regulatory mechanism remains poorly understood. Through a genetic screening of a LUCIFERASE (LUC) reporter system, we found that Arabidopsis nucleosome remodeler DECREASE IN DNA METHYL- ATION1 (DDM1) is a key component of this regulatory machinery and plays an important role in tran- scription termination, thus limiting transcriptional read-through (TRT). By whole-genome strand- specific RNA sequencing, we identified and confirmed 43 endogenous TRTs between genes, transposable elements (TEs), or genes and TEs in the ddml-10 mutant, which mainly occurred at heterochromatin regions. The DNA methylation analysis of these TRT regions revealed that TRT occurred frequently at the intergenic regions with a higher methylation level in wild type comparing to the regions where TRT did not occur. Our results suggest that the level of intergenic DNA methylation may involve in preventing aberrant gene TRT or producing new gene during evolution.

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期刊信息
  • 《遗传学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院遗传与发育生物学研究所 中国遗传学会
  • 主编:薛勇彪
  • 地址:北京市安定门外大屯路中科院遗传发育所
  • 邮编:100101
  • 邮箱:ycxb@genetics.ac.cn
  • 电话:010-64807669
  • 国际标准刊号:ISSN:1673-8527
  • 国内统一刊号:ISSN:11-5450/R
  • 邮发代号:2-819
  • 获奖情况:
  • 1996年获中科院优秀期刊二等奖,1997年获全国优秀期刊三等奖,200年获中科院优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,荷兰文摘与引文数据库,荷兰医学文摘,美国生物医学检索系统,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2000版)
  • 被引量:17519