中文摘要：

浮游藻类和营养盐的相互关系是湖沼学研究的重要内容,浮游藻类的生长受到营养盐的调节。弄清藻类的营养状态有助于理解水生植物生态,改善对水体的管理。生物测试是评价藻类营养状态的常用方法。该研究在不同温度下进行生物测试实验,对相同来源的浮游藻类分别添加不同的营养盐,对藻类生物量、碱性磷酸酶等进行监测,研究温度和营养源对浮游藻类的影响。相同的样品添加相同的营养盐在不同温度下培养,导致不同的结果;表明温度对营养盐添加试验结果影响很大,结果显示用生物测试的方法推测水体中藻类的营养状态时需尽量在原位进行,至少尽量与原位的条件一致。硝酸盐氮（NO3~-）在低温下可能成为藻类优先利用的氮源,而在25℃时氨氮（NH4~＋）为藻类优先利用的氮源。添加磷导致水体中碱性磷酸酶显著下降,而添加氮源可以导致碱性磷酸酶显著升高,表明藻类对磷的需求较大,同时氮源对碱性磷酸酶有间接影响。

英文摘要：

Phytoplankton growth is nutrient regulation, and the relationship between phytoplankton and nutrients is an important part of the study of limnology. Clarifying algae nutritional status helps to understand the ecology of aquatic plants, and improve the management of water. Bioassay is a commonly used method to evaluate the nutritional status of algae. In the pre-sent study bioassay is carded out at different temperatures, different nutrients were added to the same source of phytoplankton. To study the influence of the temperature and the source of nutrition on phytoplankton, we measured algal biomass, alkaline phosphatase, etc. The results indicate that temperature has a great influence on the results of nutrient addition. As the results show that the biological testing method speculated algae nutritional status should try to be consistent with the in situ conditions. NO3- become a priority in the use of algae as nitrogen source at low temperature, and in 25℃ when NI4^＋ became thep-riority nitrogen source of algae. Alkaline phosphatase decreased significantly after phosphorus added, nitrogen added can cause alkaline phosphatase elevated significantly, indicating that algae needs phosphorus, and indirect effects of nitrogen sources on alkaline phosphatase.