中文摘要：

以新鲜猪肺为材料，Sepharose4B偶联胰蛋白酶作为亲和层析介质，从猪肺中分离纯化高比活猪肺抑肽酶。结果表明，猪肺的抑肽酶含量为1．86mg／kg，酶比活力达4593U/mg；猪肺抑肽酶耐热性好、对pH敏感性低，在280nm处有最大光吸收，对胰蛋白酶有很强的抑制作用。

英文摘要：

The aprotintin with high activity was extracted and purified by affinity chromatography, based on crosslinking sepharose4B immobilizing trypsin.The results showed that the aprotinin yield was 1.86 mg/kg,the special activity of aprotinin reached 4 593 U/mg.The aprotinin was not only high heat-resistance,but also low pH sensitivity. And it had light absorption at 280 nm and strong inhibition to trypsin.