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利用陶瓷膜和超滤膜组合技术分离纯化天然牛磺酸
  • ISSN号:2095-6002
  • 期刊名称:《食品科学技术学报》
  • 时间:0
  • 分类:TQ028.8[化学工程]
  • 作者机构:[1]水产品深加工技术国家地方联合工程研究中心,福建厦门361021, [2]集美大学食品与生物工程学院,福建厦门361021
  • 相关基金:国家自然科学基金资助项目(31471640);国家海洋局海洋公益性项目(201305015);福建省海洋高新产业发展专项项目([2013]22号)
中文摘要:

运用陶瓷膜和超滤膜组合技术对杂色蛤(Ruditapes philippinarum)蒸煮汤汁中的牛磺酸进行分离纯化,再经浓缩、醇沉、结晶、重结晶等步骤获得高纯度天然牛磺酸。利用高效液相色谱法测定牛磺酸纯度,并对其进行扫描电镜分析和急性毒性试验。结果显示,截留孔径为0.2μm的陶瓷膜在平均压力0.24MPa、平均温度30℃、自然pH值下运行120min后,平均膜通量为32.86L/(m^2·h),牛磺酸损失率为4.99%;截留分子质量为100ku的超滤膜在平均压力0.84MPa、平均温度30℃、自然pH值下运行210min后,平均膜通量为19.97L/(m^2·h),牛磺酸损失率为5.51%。再经浓缩、醇沉、结晶处理后,可从10L杂色蛤蒸煮汤汁中获得纯度为84%的天然牛磺酸18.87g,重结晶后得纯度为98.7%的天然牛磺酸。扫描电子显微镜观测结果显示,天然牛磺酸晶体形态为长针状。急性毒性试验结果显示,天然牛磺酸的LD50〉10g/kg,属于实际无毒或无毒物质。

英文摘要:

High purity of natural taurine was isolated from cooking soup of Ruditapes philippinarum by combination of ceramic and ultrafiltration membranes, concentration, ethanol precipitation, crystallization and recrystallization. High performance liquid chromatography was used to determine the purity of taurine. Scanning electron microscopy and acute toxicity test were also performed in this study. The results showed that the membrane flux was 32. 86 L/( m^2·h) and the loss ratio of taurine was 4.99% using 0. 2μm ceramic membrane under the condition of 0. 24 MPa, 30℃, pH 7.0 for running 120 min. The ultrafiltration was performed using a 100 ku cut-off membrane with running 210 min for a cycle at 0. 84 MPa, 30℃, pH 7.0. The membrane flux and the loss ratio of taurine were 19.97 L/(m^2·h) and 5.51%, respectively. Natural taurine 18.87 g with 84% purity was finally obtained from 10 L cooking soup of R. philippinarum by the subsequent step of concentration, ethanol precipitation and crystallization. The purity of taurine could be improved to 98.7 % after recrystallization. The crystal shape of natural taurine was acicular as observed by scanning electron microscopy. Acute toxicity test in vivo revealed that the LD50 value of taurine was higher than 10 g/kg, indicating its non-mammalian toxicity.

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期刊信息
  • 《食品科学技术学报》
  • 主管单位:北京市教育委员会
  • 主办单位:北京工商大学
  • 主编:孙宝国
  • 地址:北京市海淀区阜成路33号北京工商大学
  • 邮编:100048
  • 邮箱:spxb@btbu.edu.cn
  • 电话:010-68984535
  • 国际标准刊号:ISSN:2095-6002
  • 国内统一刊号:ISSN:10-1151/TS
  • 邮发代号:
  • 获奖情况:
  • 2015年入选中国科技核心期刊2015年入选RCCSE中...
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国食品科技文摘
  • 被引量:695