中文摘要：

设计 Corynebacterium glutamicum 被破坏 argF 和 proB 的基因阻止流动离开 L 鸟氨酸为 L 鸟氨酸生产构造。L 鸟氨酸生产上的 2-oxoglutarate 脱氢酶建筑群(ODHC ) 的 inactivation 的效果也被调查。由 kgd 基因的大美人的 ODHC 的 inactivation 提高了 L 鸟氨酸生产，这被发现。设计 C。glutamicum ATCC13032 (argFproBkgd ) 生产了 L 鸟氨酸直到 4.78 g 敢 ? 湥牧獯敳 ? 湩 ? 眠吗？

英文摘要：

Engineered Corynebacterium glutamicum was constructed for L-ornithine production by disrupting genes of argF and proB to prevent the flux away from L-ornithine.Effect of the inactivation of 2-oxoglutarate de-hydrogenase complex（ODHC） on L-ornithine production was also investigated.It was found that the inactivation of ODHC by knockout of the kgd gene enhanced L-ornithine production.The engineered C.glutamicum ATCC13032（ΔargFΔproBΔkgd） produced L-ornithine up to 4.78 g·L-1 from 0.24 g·L-1 of the wild-type strain.In order to understand the mechanism of L-ornithine production in C.glutamicum ATCC13032（ΔargFΔproBΔkgd） and find out new strategies for further enhancing L-ornithine production,the comparative proteome between the wild-type and the engineered strain was analyzed.L-Ornithine overproduction in the engineered strain was related to the up-regulation of the expression levels of enzymes involved in L-ornithine biosynthesis pathway and down-regulation of the expression levels of proteins involved in pentose phosphate pathway.The overexpression of genes in the upstream pathway of glutamate to increase the availability of endogenous glutamate may further in-crease ornithine production in the engineered C.glutamicum and the ornithine synthesis enzymes（ArgCJBD） may not be the limiting enzymes in the engineered C.glutamicum.