中文摘要：

目的研究芍药苷（Paeoniflorin，Pae）对重组人白细胞介素1α（rhIL-1α）诱导的人成纤维样滑膜细胞（FLS）功能的影响。方法采用组织块培养法分离培养正常人FLS，四甲基偶氮唑蓝（MTT）法检测FLS的增殖能力，放射免疫法（RIA）检测FLS产生的TNF—α含量，酶联免疫吸附法（ELISA）检测FLS产生的PGE2和cAMP水平。结果在rhIL-1α（0．01、0．1、1、10、100μg·L^-1）的刺激下FLS增殖能力增强，产生的TNF-α和PGE，水平均不同程度升高，cAMP水平下降。Pae（10^-8、10^-7、10^-6、10^-5、10^-4mol·L^-1）体外作用可不同程度抑制由rhIL-1α诱导的FLS增殖，降低FLS产生的TNF—α和PGE2水平，升高cAMP水平。结论rhIL-1α体外刺激可以促进正常人FLS的增殖和分泌功能；Pae可以逆转rhIL-1α对人FLS功能的影响。

英文摘要：

Aim To investigate the effects of Paeoniflorin （Pae） on human fibroblast-like synoviocytes （FLS） under the stimulation of rhIL-1α. Methods The joint FLS were isolated from the healthy human and cultured as tissue culture method. The proliferation of FLS was assessed by MTT assay. Level of TNF- α was examined by radio immunoassay （RIA）, and levels of PGE2 and cAMP were determined by ELISA. Results The proliferation of FLS was stimulated by different concentrations of rhIL-1α （ 0. 01, 0. 1, 1, 10,100 μg· L^-1） , levels of TNF-α and PGE2 were increased, whereas level of cAMP was decreased. Pae （10^-8, 10^-7, 10^-6, 10^-5, 10^-4 mol· L^-1） treatment inhibited the proliferation of FLS, down-regulation of TNF-α and PGE2 levels as well as up-regulation of cAMP level under the stimulation of rhIL-1α. Conclusion Pae can reverse the proliferation and secretion function of the human FLS induced by rhIL-1α in vitro.