中文摘要：

目的探讨内脂素基因过表达对高脂诱导胰岛素抵抗（IR）大鼠胰岛素敏感性的影响。方法构建大鼠内脂素基因真核表达质粒，并用该质粒转染高脂喂养诱导的IR大鼠模型。在转染前后采用两次高胰岛素-正糖钳夹技术评价大鼠胰岛素敏感性的变化。结果成功构建了内脂素真核表达载体pcDNA3．1内脂素。实验组大鼠在pcDNA3．1内脂素质粒注射后3d血浆内脂素水平较注射前明显升高（2．19±0．36 vs 0．98±0．27，P〈0．01）；在两次胰岛素钳夹中，pcDNA3．1内脂素质粒注射后葡萄糖输注率较注射前明显升高[（32．6±1．2）mg·kg^-1·min^-1 vs （24．0±1．2）mg·kg^-1·min^-1，P〈0．01]；总胆固醇、高密度脂蛋白胆固醇则较质粒注射前明显降低[（2．36±0．22）mmol／L vs （1．60±0．21）mmol／L和（1．41±0．24）mmol／L vs（0．88±0．11）mmol／L，均P〈0．05]；在转染前后基础血糖和胰岛素水平无明显变化。结论内脂素质粒转染使IR大鼠血浆内脂素水平升高，胰岛素敏感性增强。

英文摘要：

Objective To investigate the effects of visfatin gene overexpression on insulin sensitivity in insulin-resistant （IR） rats induced by high-fat diet. Methods The recombinant visfatin plasmid was constructed and transfected into IR rats induced by high-fat diet.The euglycemic-hyperinsulinemic clamp experiments were performed for evaluation the change of insulin sensitivity before and after administration. Results The expression plasmid of visfatin were successfully constructed. After 3 days for plasmid injecting, plasma visfatin levels and glucose infusion rates were significantly increased（ 2. 19 ± 0. 36 vs 0. 98 ±0. 27 and 32. 6 ± 1.2 vs 24. 0 ± 1.2 mg·kg^-1·min^-1 , respectively, all P 〈0. 01 ）, and total cholesterol and high-density lipoprotein cholesterol （HDL-C） were significantly decreased（ 2. 36 ± 0. 22 vs 1.60 ± 0. 21 mmol/L and 1.41 ± 0. 24 vs 0. 88 ± 0. 11 mmol/L, respectively, all P 〈 0. 05 ） in high-fat diet rats. Conclusion The transfection of visfatin plasmid enhanced plasma visfatin level and improved insulin sensitivity in IR rats induced by high-fat diet.