中文摘要：

目的探讨白藜芦醇对血管紧张素Ⅱ（AngII）诱导的血管平滑肌细胞（VSMCs）增殖的影响及其可能机制。方法体外培养大鼠胸主动脉血管平滑肌细胞（VSMCs）。在检测白藜芦醇影响AngⅡ诱导VSMCs增殖和活力的实验中，将细胞分为对照组、AngII组（μmol／L）、白藜芦醇浓度梯度（ao、30、100／xmol／L）组及AngⅡ＋自藜芦醇浓度梯度组，各组细胞均反应0、6、12、24h，采用细胞计数法检测IVSMCs增殖情况，MTT法检测VSMCs活力。在检测腺苷酸活化蛋白激酶（AMPK）抑制剂复合物C对VSMCs生物活性影响的实验中，将细胞分为对照组、AngII组、白藜芦醇＋AngⅡ组及复合物C组＋白藜芦醇＋AngII组，各组细胞反应24h后采用Westernblotting检测增殖细胞核抗原（PCNA）蛋白表达。结果与对照组比较，6、12、24h后AngII组细胞活力和细胞数目均显著增高（p〈0．05）；与AngⅡ组比较，不同浓度白藜芦醇＋AngⅡ组细胞活力和细胞数目均显著降低（P〈O．05）。另一方面，与对照组比较，AnglI组PCNA蛋白表达显著增高（p〈0．05）；与AngⅡ组比较，白藜芦醇＋AngⅡ组PCNA蛋白表达显著降低（P〈0．05）；而与白藜芦醇＋AngⅡ组比较，复合物c＋白藜芦醇＋AngⅡ组细胞数目、细胞活力及PCNA蛋白表达显著增高（P〈O．os）。结论白藜芦醇可抑制AngⅡ诱导的VSMCs增殖，其机制可能与AMPK的激活有关。

英文摘要：

[Abstract] Objective To explore the effect of resveratrol on proliferation of vascular smooth muscle cells （VSMCs） as induced by angiotensin lI （Ang lI ） and its underlying mechanism. Methods VSMCs of rat＇s thoracic aorta were primarily cultured in vitro. For detecting the effects of resveratrol on Ang ][ -induced VSMC proliferation, the cultured cells were divided into a control group, Ang II group （ltxmol/L）, gradient concentrations of resveratrol groups, and Ang II ＋ gradient concentration of resveratrol groups. The gradient concentrations of resveratrol were set as 10, 30 and 100~mol/L. Cells in each group were treated for 0, 6, 12 and 24h, respectively. VSMC proliferation was detected by cell count assay, and the viability of the VMSC was measured by MTT assay. For detecting the effects of compound C [adenine monophosphate-activated protein kinase （AMPK） inhibitorl on biological effects of VSMCs, the cultured cells were divided into a control group, Ang Ⅱ group, resveratrol ＋ Ang Ⅱ group and compound C ＋ resveratrol ＋ Ang II group. The protein expression of proliferated cell nuclear antigen （PCNA） in each group was detected by Western blotting after being treated for 24 hours. Results Compared with control group, the cell viability and cell number in Ang Ⅱ group were significantly increased （P〈0.0S） after Ang Ⅱ stimulation for 6, 12 and 24 hours. Compared with Ang Ⅱ group, the cell vitality and cell number were significantly decreased in different concentrations of resveratrol ＋ Ang Ⅱ groups （P〈0.0S）. On the other hand, compared with the control group, the expression of PCNA protein in Ang Ⅱ group was significantly increased （P〈0.05）as compared with Ang I~ group, the expression of PCNA protein in resveratrol ＋ Ang Ⅱ group was significantlydecreased （P〈0.05）; as compared with resveratrol ＋ Ang H, the cell number, cell viability, and PCNA protein expression in compound C ＋ resveratrol ＋ Ang II group was significantly increased （