中文摘要：

目的 探究富含n-3多不饱和脂肪酸（ polyunsaturated fatty acid ,PUFA）的苏子油高脂饮食对肥胖大鼠肝脏极低密度脂蛋白（ very low density lipoproteins ,VLDL）合成关键基因表达的影响. 方法 造模期：雄性SD大鼠随机分为2组,对照组（normal control group, NC）给予普通日粮,高脂组（high fat group, HF）给予高脂纯合日粮诱发肥胖大鼠模型;干预期：将肥胖大鼠随机分为4组：持续高脂组（ consistent high fat group , CHF）和三个苏子油（ perilla oil, PO）替代组,根据PO替代CHF中猪油比例的不同将替代组分为20%PO、50%PO、100%PO,4周后测定大鼠血清甘油三酯（ tryglyceride, TG）水平;western blotting方法检测肝脏VLDL合成关键蛋白微粒体甘油三脂转运蛋白（microsomal triglyceride transfer protein,MTP）和载脂蛋白B（apolipoprotein B,APOB）蛋白表达;real-time PCR方法检测肝脏Mtp、Apob mRNA表达. 结果 肥胖大鼠血清TG水平显著高于NC组,并且有严重的脂肪沉积,肝脏MTP和APOB的mRNA、蛋白表达均有不同程度的降低;干预4周后,与CHF组相比,各PO替代组大鼠血清TG水平明显降低,病理切片结果显示肝脏脂肪沉积有明显改善,并且肝脏MTP和APOB的mRNA、蛋白表达均有不同程度的升高. 结论 不同比例PO替代均能促进肥胖大鼠肝脏VLDL合成与分泌,改善肝脏脂肪沉积,并且PO促进Mtp mRNA、蛋白表达具有剂量依赖性.

英文摘要：

Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the expression of key genes that regulate hepatic VLDL synthesis in obese rats .Methods Sixty healthy male 5-week old SD rats were randomly divided into 2 groups.The rats in the normal control group （NC, n=12） were given normal diet, and the rats in the high fat group （ HF, n=48） were given a pure high fat diet in order to induce rat models of obesity .In the intervention period, the obesity model rats were randomly divided into 4 subgroups including consistent high fat group （CHF） and three intervention groups depending on perilla oil substitution rate of lard in CHF：20%PO, 50%PO and 100%PO.The serum triglyceride （TG） of the rats was measured after 4 weeks.Real-time PCR was applied to measure microsomal triglyceride transfer protein （ Mtp） and apolipoprotein B （ Apob） mRNA, and western blot assay was used for detecting the expression of MTP and APOB in the liver .Results Compared with the NC group , the CHF rats exhibited significantly high fat deposi-tion.The serum TG was markedly higher and the MTP and APOB were decreased at gene and protein levels in the CHF group compared with the NC group .After the intervention , PO remarkably reduced the level of serum TG and decreased he-patic fat deposition as it showed by pathological examination .At the gene and protein levels , MTP and APOB were upregu-lated by PO to different degrees .Conclusions All the three PO intervention can promote VLDL synthesis and secretion , and decrease the hepatic fat deposition in the obese rats .Furthermore , PO upregulates the expression of MTP at gene and protein levels in a dose-dependent manner .