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中文摘要：
目的提取中药北五味子中的多糖成分，制备多糖单一组分，并测定其表观分子量。方法采用沸水浸提法提取北五味子多糖，利用DEAE-cellulose52离子交换层析，Sephacryl S-200HR凝胶层析技术对粗多糖进行纯化，得到单一组分。对所得单一组分多糖采用紫外光谱扫描和凝胶层析法进行纯度鉴定，并采用凝胶层析法；则定多糖表观分子量。结果100g北五味子提取得到粗多糖SCP5.71g，SCP经离子交换层析进一步分离后得到3个组分SCP-Ⅰ（提取率17.60％），SCP-Ⅱ（29．51％）和SCP-Ⅲ（6．30％），对主要组分SCP-Ⅰ和SCP-Ⅱ利用凝胶层析进一步纯化得到多糖单一组分SCP-Ⅰa和SCP-Ⅱa，凝胶层析法测定表观分子量分别为4．05kD和7．96kD。结论中药北五味子中主要还有两种多糖组分，SCP-Ⅰa，SCP-Ⅱa，它们的表观分子量分别为4．05kD和7．96kD。
英文摘要：
Objective To extract the polysaccharide of Sehisandra chinensis（Turcz. ） ,in prepare the single component of the pnlysaccharide,and to determine the apparent molecular weight. Methods Hot water extraction was made to extract the polysaccharide of Schisandra chinensis（Turcz. ）. Gel chromatography and inn exchange chromatography were made to purify the crude polysaceharide, and a single component was obtained. UV spectroseopy and gel chromatography were made to determine the purity of the results of single component polysaccharide. Results 5.71 g crude polysaccharide was extracted from 100g of schisandra. Three components of SCP,SCP-Ⅰ ,SCP-Ⅱ and SCP-Ⅲ were separated ＇after ion exchange chromatography. The yield rate was 17.6% ,29.51% and 6.3% for each component. The single polysaccharide components SCP-Ⅰa and SCP-Ⅱ a were purified by gel chromatography of SCP-Ⅰ and SCP-Ⅱ. The apparent molecular weight for SCP-Ⅰa and SCP-Ⅱ a was 4.05kD and 7.96kD. Conclusion The apparent molecular weights of SCP-Ⅰa and SCP-Ⅱa, the iwo main single component polysaccharide extracted from Fructus sehisandra ehinensis,were 4.05kD and 7.96kD respectively.
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