以高需冷量葡萄品种‘夏黑'为试材,石开究短日照、长日照和自然光照3个条件下,葡萄冬芽休眠的自然诱导因子及其对休眠诱导期冬芽呼吸代谢的调控机制.结果表明:自然低温、短日照2个环境因素单独或共同作用均能诱导葡萄冬芽进行自然休眠.短日照在诱导葡萄冬芽进入自然休眠的过程中起主导作用启然低温起辅助作用;温度相同条件下,日照时间越短对葡萄冬芽自然休眠的诱导作用越强.总呼吸速率达到峰值是葡萄冬芽休眠诱导期结束的标志.在自然休眠诱导期间,葡萄冬芽磷酸戌糖途径运行活性和容量占总呼吸的比例迅速上升,其中自然条件的葡萄冬芽分别由16.0%和20.1%上升至22.3%和26.0%.自然低温是诱导葡萄冬芽底物氧化水平上呼吸途径发生变化的主导因素,短日照起促进作用.在葡萄冬芽自然休眠诱导期间,交替途径运行活性和容量占总呼吸的比例迅速上升,其中自然条件葡萄冬芽分别由19.4%和27.3%上升至38.2%和46.8%.自然低温和短日照均可诱导葡萄冬芽电子传递链水平上呼吸途径发生变化.
High chilling requirement grape(Vitis vinifera—V.labrusca cv.Summer Black) was used to evaluate its dormancy under short sunlight day(SD),long sunlight day(LD) and natural condition(CK).The results indicated that grape bud dormancy could be induced by natural low temperature and short sunlight alone or together.Short sunlight was the main contributor to the dormancy of grape bud,followed by natural low temperature.SD had more effect on dormancy induction under the same temperature when compared with LD.The grape dormancy induction stopped when the total respiratory rate reached the highest level.During the dormancy induction period,the proportion of pentose phosphate pathway(PPP) operation activity or capacity to total respiratory rate increased from 16.0%to 20.1%or 22.3%to 26.0%,respectively;similarly,the proportion of operation activity or capacity of alternate pathway to total respiratory rate rapidly increased,i.e.,from 19.4%to27.3%or 38.2%to 46.8%.Both low temperature and short sunlight could induce change of respiratory pathway on electron transport chain level.