选择菲律宾蛤仔作为模式生物,研究原油分散液对海洋生物的毒理作用。挑选实验培养的健康无病害、大小均匀的菲律宾蛤仔进行48h静水法毒理试验,测定不同浓度原油分散液对菲律宾蛤仔体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化氢(H202)和丙二醛(MDA)的影响。结果表明,低浓度的原油分散液对菲律宾蛤仔有毒理兴奋作用,而高浓度的原油分散液对菲律宾蛤仔的抗氧化系统产生明显的损伤,甚至致死。石油分散液对1cm、2cm和4cm三种尺寸的菲律宾蛤仔的48h半致死浓度(Lc50)分别为43.93mg/L、46.32mg/L和48.91mg/L。
The toxicity effects of crude oil dispersion on the marine lives were investigated where P. clams were used as a model organ isms. The healthy, uniform and laboratory cultured P. clams were used in the 48 h static water toxicity test in order to evaluate 1he effect of different concentrations of crude oil on the vivo antioxidant enzymes in P. clam including superoxide dismutase ( SOD ) and cat alase ( CAT), hydrogen peroxide ( H2 02 ) and malondialdehyde ( MDA ). The results showed that low concentrations of oil dispersion were toxicological stimulant for P. clam,white high concentrations of oil dispersion caused obviously damage on the antioxidant system of P. clams, and even death. The crude oil dispersion of 48 h-LCs0 of the three sizes( 1 cm,2 cm and 4 cm) of P. clams were 43.93 rag/L,46.32 mg/L and 48.91 mg/L,respectively.