中文摘要：

目的：本实验通过检测脊髓损伤后Cdc42的蛋白表达来探讨电针结合减重步行训练疗（BWSTT）法的治疗机制。方法：选用健康成年雄性SD大鼠90只,随机分为假手术对照组（A组）、模型对照组（B组）、夹脊电针观察组（C组）、夹脊电针结合减重步行训练观察组（D组）、药物组（E组）。急性脊髓损伤（SCI）模型采用美国NYU脊椎冲击损伤仪致大鼠T9-T10段脊髓急性中度损伤。采用免疫印迹和免疫组化技术检测各时间点损伤段脊髓Cdc42的表达。结果：在两项检测技术中,假手术对照组的蛋白表达一直处于稳定低水平状态。SCI术后表达呈逐渐增加的趋势,28d时达到高峰,两组观察组及药物组与模型组相比在7d、14d、28d均有显著性差异（P〈0.05）,两组观察组之间在28d时具有显著性差异（P〈0.05）。结论：电针结合BWSTT疗法可能通过增加Rho/ROCK激酶信号通路中下游因子Cdc42的表达,促进大鼠运动功能的恢复;从损伤后长久的复健来看,电针结合BWSTT疗法对SCI后运动功能的恢复上优于电针观察组。

英文摘要：

Objective： To investigate the electric- acupunture（ EA） combined with BWSTT by evaluating the expression of Cdc42.Method： 90 adult ma Le SD rats were randomized into the sham group（ A group）,the model group（ B group）,Jia- Ji EA group（ C group）,EA with BWSTT group（ D group） and Fausidil group（ E group）. Spinal cords injury was made by NYU impactor on T9- T10 spinal segment. Immunohistochemical techniques detect the expression of Cdc42 in lesion segment at each time points. Results： Cdc42 expression stayed a low- level in the sham group. Expression after SCI was gradually increased,and reached a peak at 28 d. The two treatment groups and drug group compared with the model group had a significant differences at 7d,14 d,28d（ P〈0. 05）. The difference between the two treatment groups was significant at 28d（ P〈0. 05）. Conclusions： EA and BWSTT therapy may be increase the expression of Cdc42 to promote the recovery of motor function in rats by Rho / ROCK signa Ling pathway. For the long- term rehabilitation after SCI,the EA combined with BWSTT superior than EA group on the recovery of motor function after SCI.