中文摘要：

为探明大豆中MRP蛋白基因的作用机理，从大豆材料中克隆到GmMRP5基因完整的编码序列，GmMRP5基因的开放阅读框（ ORF）全长4479 bp，编码1492个氨基酸。序列比对与进化树分析表明：GmMRP5拥有13个推断的跨膜结构域（ TMD）和典型的ABC转运蛋白保守结构域，系统进化树分析表明，GmMRP5与AtMRP5同缘关系最近，同属于ABC转运蛋白家族C亚家族；该基因是组成型表达基因，在大豆的根、茎、叶及荚中均有表达；经不同浓度脯氨酸和柠檬酸处理后，该基因在大豆根中的表达被强烈诱导并高效表达。 Real-Time PCR结果表明，该基因在酸性缓冲液处理后表达量上调，推测其作为质子转运体参与大豆阴阳离子交换反应。

英文摘要：

In order to investigate the mechanism of the MRP protein genes in soybean ,a novel soybean gene GmMRP5 was identified in soybean cultivar.GmMRP5 containing an open reading frame ( ORF) of 4 479 bp nucleo-tides.The ORF encoded a protein of 1 492 amino acids.Sequence alignment and evolutionary tree clustering analy-sis showed that GmMRP5 contains 13 putative transmembrane-spanning domains(TMDs)and ABC transport protein conserved domain .Phylogenetic tree analysis showed that Gm MRP5 was closely related to AtMRP5 and both be-longed to ABCC subfamily .GmMRP5 was a constitutive expression gene and expressed in root ,stem and leaves by using Real-Time PCR analysis in soybean .GmMRP5 was strongly induced and highly expressed in root after treat-ment by proline and citrate solution .Real-Time PCR showed the expression level of GmMRP5 was increaced by dif-ferent pH buffer solution treatment ,suggesting that GmMRP5 gene may be as a proton transporter to participate in the stress response in soybean .