位置:成果数据库 > 期刊 > 期刊详情页
针刺对2型糖尿病大鼠胰岛素、抵抗素的作用研究
  • ISSN号:1003-5257
  • 期刊名称:《世界针灸杂志:英文版》
  • 时间:0
  • 分类:R349.64[医药卫生—基础医学]
  • 作者机构:[1]南京中医药大学针药结合实验室,南京210029, [2]山东省济宁医学院,济宁274300
  • 相关基金:国家自然科学基金资助项目(No.30772827)
中文摘要:

目的:研究针刺对2型糖尿病大鼠胰岛β细胞形态结构的作用。方法:将造模成功的大鼠按随机化原则分为针刺组、西药组、安慰剂组,同时将普食组大鼠作为正常对照组,每组10只。针刺组大鼠取"后三里"(相当于足三里)、内庭和胰俞穴给与针刺治疗,西药组用罗格列酮(0.2mg/kg体质量)灌胃,安慰剂组用双蒸水(2mL/kg体质量)灌胃,1次/d,连续4周。观察治疗前后空腹血糖、血脂变化,实验结束时,分离胰腺,石蜡包埋、酒精脱水及胰岛素(Ins)免疫组化染色后,光镜观察胰岛β细胞形态及胰岛细胞胰岛素表达。结果:针刺组胰腺腺泡、胰岛结构完整,胰岛内细胞排列大体规整,大小、形态接近正常,胞浆肿胀、纤维组织增生少见,改变显著优于西药组而接近正常组;胰岛细胞胰岛素表达针刺组显著高于安慰剂组。结论:针刺具有保护胰岛β细胞形态的作用。

英文摘要:

Objective: To study the effect of acupuncture morphologic changes of islet β-cell in type 2 diabetes rats. Methods: Model rats were divided randomly into acupuncture group (n=10), rosiglitazone control group (n=10) and placebo group (n=10). 10 male normal rats were used as normal control group. The acupuncture group rats were selected points of ‘Housanli', ‘Neiting' and ‘Yishu' to treatment with needling. The rosiglitazone group rats were given rosiglitazone (0.2mg/kg) intragastrically, the placebo group rats were given double distilled water (0.2mg/kg) intragastrically, once daily, for 4 consecutive weeks. The change of fasting blood glucose was determined before and after the treatment. Morphological change of pancrease and expression of insulin in islet β -cell were observed with light microscop. Results: Complete structure of pancreatic acinus and islet, arrangement structured of islet ceils, cells size and shape were close to normal, swelling of bubble pulp and fibroustissue hyperplasia were less see on acupuncture group. Changes of acupuncture group were significantly well than that of rosiglitazone group and close to normal control group. Insulin expression of islet cells of acupuncture group were significantly high than that of placebo group. Conclusion: Acupuncture has protective effects of morphology of islet β-cell in type 2 diabetes rats.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《世界针灸杂志:英文版》
  • 主管单位:国家中医药管理局
  • 主办单位:世界针灸学会联合会;中国中医研究院针灸研究所
  • 主编:黄龙祥
  • 地址:北京市东直门内南小街16号
  • 邮编:100700
  • 邮箱:wjam1991@sina.com
  • 电话:0086-13716997561
  • 国际标准刊号:ISSN:1003-5257
  • 国内统一刊号:ISSN:11-2892/R
  • 邮发代号:80-679
  • 获奖情况:
  • 国内外数据库收录:
  • 荷兰医学文摘
  • 被引量:398