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中文摘要：
在实验室条件下,利用溶血实验将培养于不同盐度、pH、温度和光照强度下微小卡罗藻的培养液做溶血检测,探讨不同环境因子胁迫下微小卡罗藻溶血毒素的分泌情况.结果表明：适宜盐度下该藻分泌少量溶血毒素（2hHs30=2.1%）,高盐或低盐胁迫下分泌较多（2hHs50=134.7%,2 hHs20=38.8%）.pH〈7及pH〉9时,分泌的溶血毒素较多（2 hHp5=101.7%,2 hHp10=107.4%）;pH在7～9之间,分泌很少（2 hHp7=1.5%）.高温胁迫能促进溶血毒素的分泌（2 hHt50=70.7%）,适宜温度下不分泌溶血毒素.短时间内（48h）,随着光照强度的增加（0～20000lx）,该藻的生长不受影响,溶血毒素分泌很少.因此,溶血毒素的分泌与该藻的生长环境密切相关,一旦生长受到抑制,毒素分泌量就会明显增大.
英文摘要：
The effects of different environmental stress on the Karlodinium micrum are studied by detecting the culture supernatant of the alga, which is cultured under different salinity, pH, temperature and lighting intensity. The results suggest that the Karlodinium micrum can secrete more hemolysin at high （2 hHs50=134.7%） or low （5hHs20=40.8%） salinity, but less at 30 （2hHs30=2.1%）, which is the optimal state for growing. More hemolysin （2 hHp5=101.7%, 2 hHp10=107.4%） can be secreted when pH is less than 7 or more than 9, and the hemolysin secreting of Karlodinium micrum are restrained obviously at pH in the range of 7-9 （2 hHp7=1.5%）. High temperature can accelerate hemolysin secreting （2 hHt50=70.7%）. At the normal temperature, Karlodinium micrum secretes no hemolysin. Light density has no significant effects on the hemolysin secreting for short period such as two consecutive days. With an increase in light density （0-20 000 lx）, the cells of Karlodinium micrum grows well but secretes insignificant amotmt of hemolysin. The findings in this paper indicate that there is a relation between the hemolysin secretion and growth condition. Hemolysin secretes as the growth of Karlodinium micrum is restrained.
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