中文摘要：

采用接枝二茂铁为介体的微生物传感器测量污水的BOD。将二茂铁（ferrocene，Fc）通过缩合反应接枝到大分子介孔材料SBA-15的表面用作微生物生化反应传递电子的介体，与活性污泥提取的微生物混合，并用聚乙烯醇（PVA）进行固定化，以此制备成微生物敏感膜，并与玻碳电极耦合，构建三电极传感系统，用于快速测量污水水样的BOD。结果表明，传感器的线性范围为2-300mg／L，连续测量20个样品的精密度为4．2％，能连续工作35d。并讨论了pH，温度和重金属对传感器响应的影响。通过对实际水样的测试表明，测得的BOD与BOD，的具有良好的相关性。

英文摘要：

A novel biochemical oxygen demand（BOD） detecting method employing a ferrocene（Fc） grafted SBA-15 mediator immobilized in PVA matrix was developed. Fc was combined with SBA-15 via ion-association and the product was labeled as SBA-15-Fc, which was employed for a modified glassy carbon electrode. In a three-electrode system, a linear relationship between the anodic current responses and glucose/glutamate（GGA） concentration was 2-300 mg/L. Single sensor （measuring 20 samples） reproducibility were less than 4.2 %, and the sensor can works for 35 days continuously. The effects of pH, temperature and heavy metal on the BOD responses were studied. Comparation of detecting the BOD and BOD5 of real samples showed a good correlation.