中文摘要：

以生活垃圾为原料进行好氧堆肥,采用多阶段的方式强化接种功能微生物菌剂,利用PCR-DGGE方法并结合聚类分析和Shannon-Weaver指数变化来研究堆肥过程中多阶段强化接种对细菌群落多样性的影响,同时监测木质纤维素降解率的变化.结果表明,多阶段接种堆肥能有效提高堆体降温期和二次发酵期的温度;和普通接种堆肥相比可使半纤维素、纤维素和木质素的降解率分别提高7.19%、10.89%和8.98%.细菌群落多样性分析表明,2种接种方式的多样性指数表现出极显著差异,采用多阶段的方式进行接种能使接种菌剂更好地定植于堆体之中,有效避免不同功能的接种菌剂之间、接种菌剂和土著菌之间的竞争性抑制作用,提高堆体中特别是堆肥腐熟阶段的细菌群落多样性,加快堆肥的稳定化进程.

英文摘要：

Effects of multi-stage strengthening inoculation on bacterial community diversity were analyzed using PCR-DGGE method in municipal solid waste composting combined with Cluster analysis and changes of Shannon-Weaver index,and the changes of lignocellulose＇s degradation rate were also detected during the process.The results showed that Multi-stage inoculation of composting can improve the temperature of cooling and the secondary fermentation period.And the hemicellulose,cellulose and lignin degradation rate increased by 7.19%,10.89% and 8.98% compared to general inoculation composting.The analysis of bacterial community diversity indicated that the diversity index of the two inoculation showed significant differences,the microbial inoculation could live well in the pile.It could avoid competition between different inoculated agents and competition between inoculated and indigenous microorganisms,and could improve bacterial community diversity during composting especially for maturity stage.It could speed up the composting process of stabilization.