位置:成果数据库 > 期刊 > 期刊详情页
Molecular cloning and expression profile of an abiotic stress and hormone responsive MYB transcription factor gene from Panax ginseng
  • ISSN号:1672-9145
  • 期刊名称:《生物化学与生物物理学报:英文版》
  • 时间:0
  • 分类:Q943.2[生物学—植物学] Q945.78[生物学—植物学]
  • 作者机构:[1]Molecular Biology Research Center, State Key Laboratory of Medical Genetics, School of Life Sciences, CentralSouth University, Changsha 410078, China
  • 相关基金:This work was supported by the grants from the National Natural Science Foundation of China (Nos. 30470189, 81071821, and 812111392), the Fundamental Research for the Central University of China (No. 721500011), the Key Hunan Provincial Natural Science Foundation of China (Nos. 13JJ2016 and 07JJ5096), the Science and Technology Program of Hunan Province of China (No. 20145K4050), and Changsha National High tech Zone Innovation Leading Talent Program (No. 2014-37).
中文摘要:

v-myb 鸟的 myeloblastosis 病毒的 oncogene 相当或相同的事物 MYB 家庭组成抄写因素的最丰富的组之一并且在植物在发展过程和防卫回答起重要作用。人参人参属人参 C ·迈耶·米布基因被克隆并且指定了为 PgMYB1。PgMYB1 的 cDNA 长是 762 基础对并且编码在于的 R2R3 类型蛋白质 238 氨基酸。Subcellular 本地化证明那 PgMYB1-mGFP5 熔化蛋白质在原子核是明确地局部性的。理解 PgMYB1 的功能的角色,我们在不同纸巾并且在各种各样的条件下面调查了 PgMYB1 的表示模式。量的即时聚合酶链反应和西方的污点分析证明 PgMYB1 比在茎和种子在根,叶子,和侧面的根在高水平被表示。PgMYB1 的表示由 abscisic 酸,水杨酸酸, NaCl,和寒冷是起来调整的由甲基寒心、下面调整的 jasmonate。这些结果建议 PgMYB1 可能涉及对环境压力和荷尔蒙作出回应。

英文摘要:

The v-myb avian myeloblastosis viral oncogene homolog (MYB) family constitutes one of the most abundant groups of transcription factors and plays vital roles in developmental processes and defense responses in plants. A ginseng (Panax ginseng C.A. Meyer) MYB gene was cloned and de- signated as PgMYB1. The cDNA of PgMYB1 is 762 base pairs long and encodes the R2R3-type pro- tein consisting 238 amino acids. Subcellular localization showed that PgMYBI-mGFP5 fusion protein was specifically localized in the nucleus. To understand the functional roles of PgMYBI, we investi- gated the expression patterns of PgMYB1 in different tissues and under various conditions. Quanti- tative real-time polymerase chain reaction and western blot analysis showed that PgMYB1 was expressed at higher level in roots, leaves, and lateral roots than in stems and seeds. The expression of PgMYB1 was up-regulated by abscisic acid, salicylic acid, NaCI, and cold (chilling), and down- regulated by methyl jasmonate. These results suggest that PgMYB 1 might be involved in responding to environmental stresses and hormones.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《生物化学与生物物理学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:
  • 主办单位:中国科学院上海生物化学研究所
  • 主编:
  • 地址:上海岳阳路319号
  • 邮编:200031
  • 邮箱:abbs@sibs.ac.cn
  • 电话:021-54920956 54920955
  • 国际标准刊号:ISSN:1672-9145
  • 国内统一刊号:ISSN:31-1940/Q
  • 邮发代号:4-210
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:5851