中文摘要：

利用响应面分析法对紫花苜蓿中黄酮类物质的提取工艺进行优化。先通过单因素试验，然后选取适当的试验因素与水平，并且借助微波辅助提取，最后根据中心组合试验设计，采用四因素三水平数学模型进行各个因素的显著性和交互作的分析。结果表明：紫花苜蓿黄酮醇提工艺的最佳条件为乙醇体积分数60％、料液比1比30（g·mL^-1）、微波功率450W、微波时间80S，在该条件下测得的紫花苜蓿黄酮最大提取率为81．93％。

英文摘要：

Technological conditions for microwave assisted extraction of flavonoids from Medicago sativa were optimized by the response surface testing. Single factor experiments were performed for the conditions of ① volume fraction （Ф） of ethanol for extraction; ② ratio of mass of Medicago sativa to volume of 600%（Ф） ethanol;③ microwave power; ④ time of microwave treatment and ⑤ number of times of extraction, among which factors ① to ④ were found to have more significant effect on the rate of extraction. Analysis for the interaction and significance of the relating factors was made further by applying the central composite designing principle at 4-factors and 3-levels mode. Optimum conditions found for the extraction of flavonoids were as follows：① vol. fraction （Ф） of ethanol： 60%; ② ratio of mass of Medicago sativa to vol. of ethanol： 1 to 30 （mL · g^-1）; ③ power of microwave extraction： 450 W and @ time of microwave assisted extraction： 80 s. The rate of extraction achieved to a max. value of 81.93% under the optimum conditions.