中文摘要：

以便在猪检测杂种优势的分子的基础，抑制减少性的杂交被执行在在 MeishanxYorkshire F1 生气品种和他们的父母，之间的 Longissimus 欧洲粪金龟子 i 肌肉纸巾在基因表示调查差别 Meishan 猪。猪肌浆球蛋白规章的轻链 2 (MRLC2 ) 在生气品种和 purebreds 之间表示的基因差别被孤立并且用半量的反向的 transcriptase 鉴别聚合酶链反应和它的完全的 cDNA 顺序用 cDNA 结束的快速的扩大被获得方法。基因的核苷酸顺序不对任何已知的猪的基因相应。顺序预言分析表明这基因的开的读物框架编码包含 EF 手总科的通常认为的保存领域的 172 氨基酸的蛋白质。猪的 MRLC2 蛋白质的这个预言的氨基酸序列展出 99% ， 98% ， 98% ， 98% 和 97% 有牛，人，狗，老鼠和老鼠的身份分别地。相同分析表明 MRLC2 蛋白质非常在进化被保存。组织表示分析显示猪 MRLC2 基因高度在肌肉，脂肪，心，肝，脾，肺，肾，胃，小肠，卵巢和睾丸被表示，然而并非在胰表示了。

英文摘要：

In order to detect the molecular basis of heterosis in pigs, suppression subtractive hybridization was carried out to investigate the difference in gene expression in the Longissimus dorsi muscle tissues between MeishanxYorkshire F1 crossbreeds and their parents, Meishan pigs. The swine myosin regulatory light chain 2 （MRLC2） gene differentially expressed between the crossbreeds and the purebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reaction and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acids containing the putative conserved domain of the EF-hand superfamily. This predicted amino acid sequence of porcine MRLC2 protein exhibits 99%, 98%, 98%, 98% and 97% identity with that of cattle, human, dog, rat and mouse, respectively. The homology analysis revealed that the MRLC2 protein was very much conserved in evolution. The tissue expression analysis indicated that the swine MRLC2 gene is highly expressed in muscle, fat, heart, liver, spleen, lung, kidney, stomach, small intestine, ovary and testis, but not expressed in pancreas.