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中文摘要：
无细胞蛋白表达体系是一种以细胞抽提物为基础的体外合成蛋白质表达技术，具有遗传背景简单、反应操控简便等特点，已成为研究生物反应系统的重要技术手段。在研究人员的不断努力下，反应体系从原核扩展到真核蛋白质合成体系，而且目标蛋白表达量从毫克级提高到数克级每升，成本不断降低，反应规模可达到百公升级。近年来，无细胞蛋白表达系统在复杂蛋白、毒性蛋白和膜蛋白表达方面的优势逐渐体现，展示了其在生物制药领域的重要应用潜力。总之，无细胞技术已经成为异源蛋白质高效合成和生物制药领域中有巨大潜力的新策略。
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