中文摘要：

目的：利用Micro-CT对使用福尔马林进行固定并浸泡了碘对比剂的鼠脑进行无损的离体成像,以此来区分其灰质和白质,甚至一些更细微的鼠脑部结构,为后续鼠脑的研究提供一些实验依据。方法：成像之前,分离出鼠脑的离体样本,并分成两组,将其中一组浸泡在福尔马林溶液中进行固定处理,另外一组先浸泡福尔马林溶液（48h）后再浸泡入碘对比剂溶液中（21d）。而后对鼠脑进行Micro-CT成像,利用成像后的数据进行三维重建,获得能从各个方向进行观察的Micro-CT三维离体鼠脑成像。结果：浸泡了碘对比剂的鼠脑成像相比于未经碘对比剂浸泡的鼠脑成像,成像后脑的灰白质之间的对比度要好得多,一些细微的鼠脑结构也更为清晰。其中,碘对比剂对灰质的对比度增强效果最好,对周围的白质对比度增强效果居中,对密集的白质束纤维,如胼胝体、扣带、外囊、前连合的增强效果最小。结论：Micro-CT成像可以用于浸泡了碘对比剂后的离体鼠脑结构成像,并最终获得直观、立体的三维鼠脑成像。

英文摘要：

Objective To distinguish the gray matter and the white matter of rat brain by nondestructive imaging of rat brain invitro through micro-CT based on iodine contrast medium. Methods Before imaging, the rat brains were isolated and weredivided into two groups. One group was post-fixed in formalin, the other group was post-fixed in the formalin for 48 hours thensocked in iodine contrast medium for 21 days. All rat brains were scanned by micro-CT and three-dimensional images of ratbrain from all directions can be obtained. Results Compared with the rat brains without iodine contrast medium, the rat brainswith iodine contrast medium can produce better contrasts between the gray and the white matters. Some tiny structures of the ratbrains are more clear. More importantly, the contrast enhancement of the gray matter is the best and the white one would be inthe middle. As for of the corpus callosum, cingulum, external capsule and anterior commissure, the contrast enhancements areleast. Conclusion The present results demonstrate that micro-CT combined with iodine contrast medium can be used forthree-dimensional imaging and the exploration of the structure of rat brain in vitro. And, the direct and three-dimensionalimages of rat brain can be obtained.