中文摘要：

目的 观察青春期和成年早期小鼠经饮水暴露铅和镉对雄性生殖功能的影响。方法 健康4周龄雄性ICR小鼠被随机分入对照组、铅组、镉组、铅＋镉组,每组15只。各组小鼠分别经口饮用纯净水（反渗透水）、醋酸铅水溶液、氯化镉水溶液以及醋酸铅和氯化镉混合水溶液,暴露40d后摘眼球取血,处死小鼠后取睾丸、附睾,称脏器重量,计算脏器系数,进行附睾尾精子计数。HE染色观察睾丸组织形态学改变,用酶联免疫吸附法检测小鼠血清睾酮含量。结果 与对照组比较,铅组、镉组和铅＋镉组小鼠的饮水量、进食量和体重增长差异无统计学意义。染毒40d后,单纯铅、单纯镉暴露或镉铅联合暴露均未改变小鼠体重、睾丸、附睾重量及其脏器系数。进一步研究表明,与对照组比较,铅组、镉组和铅＋镉组小鼠附睾精子浓度和血清睾酮均差异均无统计学意义。结论 本实验条件下,青春期和成年早期联合暴露铅与镉未引起小鼠雄性生殖损害作用。

英文摘要：

Objective To observe the effects of lead and cadmium co-exposure through drinking water during pu- berty and early adulthood on male reproduction in mice. Methods Healthy male ICR mice at 4 weeks were ran- domly divided into the control, lead, cadmium, lead plus cadmium group. There were 15 mice in each group.Mice in each group were exposed to pure water （reverse osmosis water）, lead acetate solution, cadmium chloride solution, and the mixed solution including lead acetate and cadmium chloride. After exposure of 40 days, blood was collected by removing eyeballs, and then mice were killed. The testes, epididymides were detached and weigh- ted. The organ coefficient of testes and epididymides was counted. Sperm was counted from cauda epididymidis. Testicular histological morphology was evaluated using H＆E staining. Serum testosterone concentration was ana- lyzed using enzyme linked immunosorbent assay （ELISA）. Results No significant difference in water consump- tion, diet intake, and body weight gain was observed among cadmium, lead, cadmium plus lead co-exposure group and the control group. After exposure of 40 days, lead, cadmium or lead plus cadmium did not cause the change of body weight, the weight of testes and epididymides, and organ coefficient of testes and epididymides. Further re- search showed that no obvious difference in sperm count and serum testosterone concentration was observed among the four groups. Conclusion The present study shows that lead and cadmium co-exposure during puberty and ear- ly adulthood does not affect male reproduction in mice.