利用形态标记和SSR分子标记技术分析了澳L23/757(鸡脚叶)、中棉所19(正常叶)、红桃(红叶)的25、50、100、150株不同自然授粉繁殖群体的遗传完整性.结果表明,3个种质间的形态标记的天然异交率和SSR标记遗传纯度有显著差异,但4个群体大小间的天然异交率和SSR标记遗传纯度的差异均不显著.说明棉花繁殖群体为25株时,可作为棉花种质繁殖更新过程中的最低繁殖群体量,能较好地保持繁殖种质的遗传完整性.
The genetic integrity of different natural pollination populations with 25, 50, 100, and 150 plants in three germplasms of AoL23/757 (okra-leaf), CCRI 19 (normal-leaf) and Hongtao (red-leaf) was analyzed using morphological characteristics and SSR markers. The results showed that both the natural outcrossing rates of morphological markers and the molecular genetic pu- rity of SSR markers among the three germplasms were significantly different, but the four breeding population sizes did not dis- play significant differences. This proved that 25 plants could be used as the lowest regeneration population size, for efficient maintenance of the genetic integrity of cotton germplasms (Gossypium hirsutum L.).