中文摘要：

目的 观察骨髓间充质干细胞（mesenchymal stem cell,MSC）移植对Tourette综合征（Tourette syndrome,TS）大鼠刻板行为的改善及体内多巴胺（dopamine,DA）含量的影响.方法 48只Wistar大鼠被分成假模型组、模型组和MSC移植组（每组n=16）,建立TS大鼠自身免疫动物模型,采用立体定向脑内注射法将BrdU标记的MSC移植到TS大鼠纹状体内,观察大鼠刻板行为改善情况.通过高效液相色谱-电化学法检测大鼠纹状体及血浆中的DA含量.结果 MSC移植组大鼠在细胞移植后7d、14d和28d的刻板行为总分[分别为7d：（117.3±8.4）分,14d：（94.8±7.9）分,28d：（78.8±7.4）分]及纹状体多巴胺含量[分别为7 d：（898.2±143.2）ng/g,14d：（812.4±92.2） ng/g,28d：（757.9±82.4） ng/g]均低于模型组[分别为7d：（129.3±9.8）分,14d：（116.5±8.5）分,28 d：（106.8 ±6.4）分;7d：（1209.3±182.3） ng/g,14d：（1033.5±114.7） ng/g,28 d：（994.8±112.6） ng/g,P＜0.01],在MSC移植后7d、14d移植组大鼠血浆DA 的含量低于模型组[分别为7 d：（530.2±86.2） ng/g,14 d：（562.4±64.2） ng/g,7d：（690.3±72.7）ng/g,14d：（689.4 ±83.9）ng/g,P＜0.01].结论 MSC移植能减少TS大鼠的刻板行为,影响多巴胺的代谢.

英文摘要：

Objective To investigate the effect of mesenchymal stem cell transplantation on stereotypic behavior and level of dopamine of Tourette syndrome（TS） rats.Methods Wistar rats were randomly divided into three groups namely sham group （microinfused with normal sera）,TS group （microinfused with TS sera）group and TS plus MSC transplantation group.Auto-immune TS rat model were established and MSC suspension labeled with BrdU was injected into the striatum of TS rats.DA were measured by high performance liquid chromatography and electrochemical detection.Results TS rats with MSC grafts exhibited significantly decreased stereotypic behaviors （7d：117.3 ±8.4,14d：94.8 ±7.9,28 d：78.8 ±7.4）and level of DA in striatum（7 d：（898.2 ± 143.2） ng/g,14d：（812.4 ±92.2）ng/g,28 d：（757.9 ± 82.4）ng/g） at 7,14 and 28 days after transplantation compared to model group（ （ 129.3 ± 9.8,116.5 ± 8.5,106.8 ± 6.4 ; （ 1209.3 ± 182.3 ） ng/g,（ 1033.5 ± 114.7 ） ng/g,（994.8 ±112.6） ng/g,P〈0.01）.DA in plasma of TS rats with MSC grafts also decreased at 7 and 14 days（7 d：（530.2 ±86.2）ng/g vs （690.3±72.7） ng/g;14d：（562.4 ±64.2） ng/g vs （689.4±83.9） ng/g,P〈0.01）.Conclusions Transplantation of MSC could reduce stereotypic behaviors in TS rats and regulate the metabolism of DA.