中文摘要：

安徽虫瘟霉Zoophthora anhuiensis（Li）Humber是较难人工培养的蚜虫专化性病原真菌.将灭菌并适度熟化的黍米Panicum miliaceum L.作为固体基质与挑碎的安徽虫瘟霉平板菌落混合,在20℃和12L：12D的温光条件下静止固体培养,获得了产孢潜能大、杀蚜活性强的米粒培养物.培养7天的黍米的产孢量达13.0×10^4个孢子/粒,产孢持续时间长达6天.用此黍米培养物弹射的孢子对桃蚜Myzus persicae（Sulzer）若蚜进行7.9～134.9个孢子/mm^2共9个剂量的孢子浴接种,所获数据很好拟合时间-剂量-死亡率模型.接种后第5～7天各天的LC50依次为59.8,39.5和33.5个孢子/mm^2,LC90依次为354,234和198个孢子/mm^2.在57.7～134.9个孢子/mm^2的接种剂量范围内,致死中时LT50从5.1天下降到4.3天.由此表明,安徽虫温霉的黍米培养不仅简单易行,而且菌种的产孢和侵染生物学特性在培养物中被充分体现,每颗米粒如同自然罹病而死的蚜尸,值得进一步研究开发和利用.

英文摘要：

The obligate aphid pathogen, Zoophthora anhuiensis （Li） Humber （Entomophthorales）, is difficult to be propagated in vitro. In this study, the broomcorn millet Panicum miliaceum L. in flasks was used as solid substrate to ease in vitro propagation of the funsal pathogen. Properly steamed millet grains in flasks were mixed with pieces of the fungal colonies grown on the plates of Sabouraud egg yolk-milk agar and then maintained for stationary incubation at the regime of 20℃ and 12L ： 12D. The 7-day-old millet cultures produced 13.0 × 10^4 conidia/grain and persistently sporulated for up to 6 days. The conidia discharged from the cultured grains were used to inoculate via spore shower the nymphs of the green peach aphid Myzus persicae （Sulzer） on cabbage leaves at nine dosages of 7.9 - 134.9 conidia/mm：. Data resulted from 7-day observation of the inoculated aphids fit very well to the time-dose-mortality model. Based on time- and dose-effect parameters estimated from the modeling, the LC50 and LC90 of the fungus against M. persicae were 59.8 and 354 conidia/mm： on day 5 after spore shower, 39.5 and 234 conidia/mm： on day 6, and 33.5 and 198 conidia/mm： on day 7, respectively. The LT50 decreased to 4.3 days at the dose of 134.9 conidia/mm^2 from 5.1 days at 57.7 conidia/mm^2. The results showed that the millet method for propagation of the obligate aphid pathogen was not only easy and convenient but produced desirable millet cultures with each grain sporulating very well and infecting aphids as a natural host cadaver.