中文摘要：

目的：观察蝎毒多肽B5（SVPB5）对正常及辐射后的大鼠骨髓间充质干细胞（r BMSCs）生长的影响。方法：利用全骨髓细胞贴壁法分离纯化r BMSCs,体外扩增传代,取第3~5代细胞为实验对象。实验分为正常对照组（IR-control）、正常加药组（IR-SVPB5）、辐射对照组（IR＋control）以及辐射加药组（IR＋SVPB5）。分别进行细胞计数、衰老相关β半乳糖苷酶染色及集落形成单位计数。结果：SVPB5对正常及辐射r BMSCs均有促进增殖的作用,其中SVPB5处理3 d后,对正常r BMSCs开始便表现为明显的促增殖作用（P〈0.05）,而对辐射r BMSCs则于第10、11天表现出明显的促增殖作用（P〈0.05）。SVPB5作用于正常r BMSCs后,其衰老染色阳性细胞率明显低于正常对照组（P〈0.05）,其集落数明显高于正常对照组（P〈0.05）。结论：SVPB5能促进正常及辐射后r BMSCs增殖,增强r BMSCs的增殖潜能并缓解其衰老。

英文摘要：

Objective： To observe scorpion venom peptide B5（ SVPB5） on growth of normal and irradiated rat bone marrow-derived mesenchymal stem cells（ r BMSCs）. Methods： r BMSCs were separated and purified with adherent cultivation of whole bone marrow cells,then cultured and amplified in vitro. Passage 3-5 r BMSCs were used as experimental subjects,divided into normal control group（ IR-control group）,normal dosing group（ IRSVPB5 group）,irradiation control group（ IR ＋ control group） and the irradiation dosing group（ IR ＋ SVPB5group）. Each group was counted for total cells,colony forming units and stained with senescence-associated β-galactosidase. Results： SVPB5 promoted proliferation of both normal and irradiated r BMSCs. Specifically,the proliferation-promoting effect became obvious as early as 3d of SVPB5 treatment in normal r BMSCs（ P 〈 0. 05）,which was not shown in irradiated r BMSCs until 10-11 d of the treatment（ P 〈 0. 05）. After SVPB5 treatment,the normal r BMSCs showed significantly lower percentage of senescence β-galactosidase staining positive cells and higher counts of colony forming units,compared with the normal control group（ both P 〈 0. 05）. Conclusion：SVPB5 may promote proliferation of normal and irradiated r BMSCs,enhance proliferation potential and mitigate cell aging of r BMSCs.