目的对诱导白木香产生沉香的3株活性真菌的固体培养条件进行优化,为该类真菌的推广应用提供参考。方法采用真菌固体培养方法,对影响真菌生长的营养和培养条件等因子进行优化,实验结果采用单因素方差分析法。结果得到了培养3株活性真菌的最佳固体培养基配方,获得了真菌固体生长的最适温度、pH值、装料量、基质含水量和光照条件。结论在实验得到的最佳条件下,能够较快的培养出高质量的固体菌种;为3株活性真菌诱导白木香产生沉香的生产推广提供了菌种培养的优化条件。
OBJECTIVE To optimize the solid culture condition of three fungi which can induce Aquilaria sinensis to produce agilawood, thus to provide data for the production and application of the fungi. METHODS Single factor experiment method was applied in the study. RESULTS The optimal solid culture medium formula, temperature, pH value, moisture, the density of materials and illumination conditions for three fungi were obtained. CONCLUSION The optimized method can provide high quality fungi for prompting Aquilaria sinensis to produce agilawood at larger scales.