以黄色和浅黄色钝裂银莲花(Anemone obtusiloba)花瓣为研究材料,对其色素进行特征显色反应和紫外-可见光谱扫描分析,并对其色素的成分及其稳定性进行研究。结果显示,两种花色钝裂银莲花花瓣均含有叶绿素和类胡萝卜素,主要色素为黄酮类化合物(花色素苷、黄酮、黄酮醇和异黄酮),不含橙酮和二氢黄酮,其黄酮类化合物均表现为酚羟基结构,可能具有4-酮基、3-OH或4-酮基、5-OH结构的特征;p H值对花色素具有一定影响,避光保存花色苷的稳定性强于光照,Fe3+和Cu2+对花色素稳定性影响比较大。
The components and stability of pigment from Anemone obtusiloba with two different flower colors were analyzed by the specific color reactions and UV-visible spectrum. Results showed that the two different colored petals of A. obtusiloba consisted of chlorophyll and carotenoid,the main petal pigments belonged to flavonoid,definitely including flavone,anthocyanins,flavonols,flavanonols,excluding flavanonols and aurones. These flavonoid had phenolic hydroxyl,4- keto,3- hydroxy or 4- keto,5- hydroxy. The stability of anthocyanins was affected by different values of p H,the anthocyanins under dark condition were more stable than that under light. The stability of petal pigments was mainly influenced by Fe^3 +and Cu^2 +.