中文摘要：

为了探讨不同形态磷酸盐对铜绿微囊藻（Microcystis aeruginosa）和蛋白核小球藻（Chlorella pyrenoidosa）藻细胞生长的影响，采用3种不同形态的磷酸盐：磷酸氢二钾（K2HPO4），焦磷酸钠（Na4P2O7）和一聚磷酸钠（Na5P3O10）作为BG-11培养基中的添加磷源．从两种藻的生长趋势可以看出，在K2HPO4培养基中小球藻在生长初期生长速度显著高于微囊藻，当小球藻的生长到达静止期时，微囊藻的生长速度便超过了小球藻且其藻密度也高于小球藻；而在焦磷酸钠和二三聚磷酸钠培养基中，小球藻的生长速度要显著高于微囊藻．同时微囊藻和小球藻在K2HPO4培养基中的生长速度则始终高于另两种磷酸盐培养基中的生长速度．藻细胞碱性磷酸酶活性变化曲线也显示，小球藻的磷利用能力要高于微囊藻．由此可见，小球藻对磷的吸收利用能力高于微囊藻，从而表明铜绿微囊藻成为水华优势种并不是取决于其对磷酸盐的吸收利用能力。

英文摘要：

To explore the effects of different types of phosphate on the growth of Microcystis aeruginosa and Chlorella pyrenoidosa, three types of phosphate salts： dipotassium hydrogen phosphate （K2HPO4）, sodium pyrophosphate（Na4P2O7） and sodium polyphosphate （Na5P3O10） were used as phosphate sources in this experiment. The growth curves showed that, at early stage of growth in the K2HPO4 medium, the growth rate of C. pyrenoidosa was significantly higher than that of M. aeruginosa, but on the 16th day, the growth rate of M. aeruginosa and its cell density were all higher than those of C. pyrenoidosa. The growth rate of M. aeruginosa and C. pyrenoidosa in K2HPO4 medium were significantly higher than those in other two phosphate media. Both in the media of sodium pyrophosphate and sodium polyphosphate, the growth rate of C. pyrenoidosa was higher than that of M. aeruginosa. The changes of tmicell alkaline phosphatase （APA） suggestted that, the phosphate utilization of C. pyrenoidosa was higher than that of M. aeruginosa. The results suggestted that the phosphate utilization capability of C. pyrenoidosa was higher than that of M. aeruginosa, indicated that phosphate utilization was not the main cause which resulted in the dominance ofM. aeruginosa in water-bloom formation.