中文摘要：

为了揭示小麦TaSnRK2.9基因的结构特点和功能特性,为该基因的研究和应用提供基础,根据已报道的水稻蛋白激酶SAPK9基因（GenBank登录号AB125310.1）序列信息,利用RT-PCR技术克隆了小麦TaSnRK2.9基因,并对其进行生物信息学分析。结果表明：小麦TaSnRK2.9阅读框为1092bp,编码363个氨基酸,与水稻SAPK9亲缘关系最近,属于第三亚家族;亚细胞定位预测TaSnRK2.9定位在细胞质中;基因表达谱分析发现TaSnRK2.9在根、茎、叶、花等器官和组织中都有表达,并且在叶中的表达量最高。TaSnRK2.9的克隆为研究其在小麦中的功能提供基本信息和指导。

英文摘要：

In order to reveal the structure and function characteristics of TaSnRK2.9 in wheat, and provide the basis for the research and application of this gene, the cDNA of TaSnRK2.9 was cloned in wheat by RT-PCR based on the nucleotide sequence of SAPK9 gene in rice, and the bioinformatics analysis was also finished. The results showed that the length of ORF was 1092 bp, encoding 363 amino acids. Sequence analysis and the phylogenetic trees showed that this gene was closely related to SAPK9 （AB125310） of rice, belonged to the subfamily Ⅲ. Subcellular localization predicted TaSnRK2.9 gene located in the cytoplasm. Gene expression profile analysis found that TaSnRK2.9 gene expressed in roots, stems, leaf, flowers, and was more highly expressed in leaf. The cloning of the TaSnRK2.9 gene full length cDNA provided the basis and guidance for its characteristics and function.