中文摘要：

4克隆藏羚羊血红素氧合酶-1基因编码区，并进行序列分析。方法：从藏羚羊肝组织中提取总RNA，利用逆转录聚合酶链反应（RT-Pea）技术扩增出血红素氧合酶-1（HO-1）基因编码区eDNA片段，与载体连接构建重组质粒，经转化、扩增培养、鉴定后测序，测序结果利用生物信息学的方法进行分析。结果：藏羚羊HO-1基因编码区长度为897，编码298个氨基酸（GenBank登录号为：JQ809687）；序列分析表明，藏羚羊HO-1基因的编码区序列与脊椎动物山羊和牛的相似性分别达到98％和96％，氨基酸序列相似性分别达到92％和97％，与其它脊椎动物HO-1基因的核苷酸及氨基酸序列相似性达到86％和87％以上，显示出高度保守性。构建的基于氨基酸序列的分子系统进化树聚类结果表明，藏羚羊与山羊的进化距离最近。结论：本研究成功地克隆出藏羚羊110-1基因编码区序列，为从低氧细胞保护角度探讨青藏高原土著物种适应高原的分子生物学机制研究提供实验依据。

英文摘要：

To clone and analyze the encoding region of heme oxygenase - 1 （ HO - 1 ） gene from Tibet- an antelope （Pantholops hodgsonii）. METHODS ： The total RNA was isolated from the liver of Tibetan antelope and the HO - 1 gene was amplified by RT - PCR. The PCR product was cloned into pGEM - T vector and sequenced. The nucleo- tide sequences were compared with the data from GenBank by BLAST method. RESULTS ： The encoding region of 1t0 - 1 gene from Tibetan antelope was obtained and deposited in GenBank as accession number JQ809687. The encoding sequence was 897 bp in full length, which encoded 298 amino acids. Sequence similarity analysis showed that the HO - 1 gene cloned from Tibetan antelope shared 98% and 96% in the nueleotides, and shared 92% and 97% in the amino acids with those of Capra hircus and Bos taurus, respectively. The sequence similarity of nucleotides and amino acids also shared 86% and 87% with other vertebrates, which were highly conservative. The molecular phylogenetic tree based on the amino se- quence of HO - 1 showed that Tibetan antelope and Capra hircus was classified to one cluster, which was basically concert- ed to the evolutionary relationship of traditional species. CONCLUSION： The encoding region of Tibetan antelope HO - 1 gene is successfully cloned, which provides a foundation for exploring the molecular and biological mechanisms of high alti- tude adaptation and cell protection in Qinghai -Tibet Plateau species under hypoxic condition.