中文摘要：

应用荧光显微技术、激光共聚焦扫描显微技术、单克隆抗体免疫荧光标记技术以及傅里叶变换显微红外光谱分析（FTIR）等手段，研究了内钙拮抗剂TMB-8对白皮松花粉管胞内Ca2＋分布、花粉管生长以及细胞壁构建等的影响。结果表明，白皮松花粉管经TMB-8处理后，胞内的ca2＋浓度下降，花粉管内典型的ca2＋浓度梯度消失，花粉萌发和花粉管生长均受到不同程度的抑制，抑制效果与拮抗剂浓度呈正相关。在对照中，纤维素和胼胝质在花粉管细胞壁上均匀分布，酸性果胶质仅分布在花粉管管壁上，而酯化果胶质仅分布于花粉管顶端；经TMB-8处理后，花粉管顶端纤维素、胼胝质以及酸性果胶质含量增加，而酯化果胶质含量减少。这些表明胞内ca2＋对花粉管顶端ca2＋浓度梯度的建成具有重要的作用，抑制胞内ca2＋释放可以影响花粉管细胞壁的结构组成和物理性质，从而抑制花粉管的生长。

英文摘要：

The effects of TMB-8,an intracellular calcium antagonist,on the pollen tube growth and cell wall construction in Pinus bungeana were investigated by means of fluorescence microscopy,laser scanning confocal microscopy,immunofluorescence technique and Fourier transform infra-red（FTIR） microspectroscopy.The results showed that TMB-8 inhibited P.bungeana pollen germination and tube growth in a dose-dependent manner.Normally growing pollen tubes displayed a typical tip-focused cytosolic free Ca2＋ concentration gradient,while the Ca2＋ concentration gradient dissipated in TMB-8-treated pollen tubes.Cellulose and callose were distributed uniformly in the wall of pollen tubes;acidic pectin was distributed in the tube wall except for the apex while esterified pectin was present only in the apex.In TMB-8-treated tubes,the content of cellulose,acidic pectin and callose increased while esterified pectin decreased in the apex compared with the normal tube wall.It indicated that the intracellular Ca2＋ was necessary in the formation of apical cytosolic Ca2＋ concentration gradient.Inhibition of intracellular Ca2＋ release resulted in the arrest of pollen tube growth by the modification of cell wall composition in P.bungeana pollen tube.