中文摘要：

以大豆为试验材料,研究了干旱胁迫下NO对抗氧化酶活性的调节作用。结果显示,干旱胁迫处理显著地增强了大豆根中NO产生和抗氧化酶SOD、CAT和POD活性;外源NO处理进一步增加了抗氧化酶活性,而NO清除剂处理则抑制了NO产生和抗氧化酶活性;NO合成酶（NOS）抑制剂和硝酸还原酶（NR）抑制剂处理均明显抑制了干旱胁迫下NO产生和抗氧化酶活性,且NOS抑制剂影响程度更大。此外,干旱胁迫增强了NOS和NR活性,而且NOS活性增幅比NR大。这些结果表明,干旱胁迫下NOS和NR途径介导的NO产生参与了大豆根中抗氧化酶活性的调节,而且NOS途径起着主要作用。

英文摘要：

Soybean was used to investigate the regulatory role of NO on the activity of antioxidative enzymes in soybean seedlings under drought stress.The results showed that NO production and activities of SOD,CAT and POD were significantly increased in the soybean roots exposed to drought stress.Application of exogenous NO to soybean seedlings further increased the activity of antioxidative enzymes,while NO scavenger inhibited NO production and the activity of antioxidative enzymes.Both inhibitors of NO synthase（NOS） and nitrate reductase（NR） significantly inhibited NO production and the activity of antioxidative enzymes under drought stress,and NOS inhibitor had greater effect than NR inhibitor had.The activities of NOS and NR were increased under drought stress,and NOS activity was more than NR activity.The results indicated that both NOS and NR mediated NO production was involved in regulating the activity of antioxidative enzymes under drought stress.NOS pathway played a dominant role in this process.