中文摘要：

合成了一种新的硒二唑衍生物4-（苯并[c][1,2,5]硒二唑-6-yl）苯-1,2-二氨基（BSBD）.研究了其抗肿瘤活性,结果发现BSBD具有很好的抗癌活性,特别对Neuro-2a小鼠脑神经瘤细胞表现出很好的选择性.关于作用机制的研究发现,BSBD可呈剂量效应的诱导肿瘤细胞中Sub-G1凋亡峰的累积、染色质固缩及凋亡小体的形成,说明诱导细胞凋亡是BSBD发挥抗肿瘤活性的主要机制.进而应用紫外-可见吸收光谱、荧光光谱法、傅里叶红外光谱、圆二色光谱法研究了其与牛血清白蛋白（BSA）的相互作用.采用位点结合模型公式和热力学公式计算了结合常数、结合位点数及结合力类型,并用紫外-可见吸收光谱、红外光谱和圆二色光谱技术探讨了硒杂环化合物对牛血清白蛋白构象的影响.结果表明：BSBD能有效淬灭牛血清白蛋白的荧光强度,其猝灭机理为静态猝灭,主要作用力类型是氢键和范德华力.而同步荧光、紫外-吸收示差光谱、红外光谱和圆二色光谱都进一步证明了BSBD与BSA结合后,改变了BSA的内部结构.

英文摘要：

A novel selenadiazol derivative （BSBD） has been synthesized, characterized and evaluated for its anticancer activity. The in vitro anticancer activities of BSBD were screened by MTT assay against various cancer cell lines and it was found that BSBD could effectively inhibit cancer cell growth, especially for Nuero-2a neuroblastoma cells. Further investigation on the action mode showed that BSBD could induce Sub-G1 peak accumulation, chromatin condensation and the formation of apoptotic body in a dose-dependent manner in cancer cells, indicating that induction of apoptosis is the main mechanism accounting for the action of BSBD. Moreover the interaction between BSBD and bovine serum albumin （BSA） was investigated by UV-Vis, fluorescence and circular dichroism spectrometry and FTIR. The binding constants, number of binding sites and type of binding force were calculated by applying the equation of site-binding model and thermodynamic equations. The results of UV-Vis, FTIR and circular dichroism spectrometry revealed that the major interaction mode between BSA and BSBD was static quenching, due to hydrogen bonding and van der Waals force. The results of spectroscopic characterization showed BSBD altered the chemical structure and conformation of BSA.