中文摘要：

为研究可溶性人TRAIL蛋白（sTRAIL）对肝癌细胞株SMMC7721的生长抑制效应及凋亡诱导作用．采用显微镜、台盼蓝排斥试验、MTT比色试验、TUNEL法和DNA断裂实验等方法检测细胞增殖和细胞凋亡．通过显微镜观察到核染色质凝集等凋亡的形态学变化，台盼蓝排斥试验、MTT比色试验结果显示，sTRAIL蛋白可显著抑制SMMC7721细胞的生长和增殖，并且TUNEL法检测到经sTRAIL处理后的细胞凋亡指数与对照比较有显著差异，DNA断裂实验亦观察到典型的DNA梯形条带，这些结果提示sTRAIL可诱导肝癌细胞株SMMC7721发生凋亡，具有抗肝癌的作用．

英文摘要：

To investigate the effects of human soluble TRAIL（sTRAIL） on cell growth and apoptosis of hepatocellular carcinoma cell line SMMC7721. After treatment of sTRAIL, the cell proliferation and apoptosis are determined by invertible microscope , trypan blue exclusion test, MTr assay, terminal deoxynucleotidly transferase mediated labeling （TUNEL） and DNA fragmentation assay. Chromatin condensation is observed by invertible microscope, Proliferation of SMMC7721 cell is significantly inhibited after the treatment with sTRAIL,TISNEL results show in comparison with control, the difference of the apoptosis index of ceils treated by sTRAIL is significant , and DNA ladder is observed. The results indicate that sTRAIL can induce apoptosis of hepatocellular carcinoma cell line SMMC7721.