中文摘要：

利用珠磨器和不锈钢珠，以甘蔗及其近缘属割手密、斑茅和河八王为材料提取基因组DNA，并对得到的DNA进行电泳检测、含量测定和SSR分析。结果表明，该方法能高效快速提取基因组DNA，所提取DNA中蛋白质、多糖类物质等去除较彻底，DNA纯度较高，完整性好；SSR分析条带清晰，多态性较丰富，能扩增出特异性谱带。该方法无需液氮研磨，简单、快速、高效，经济成本低、PCR扩增效果好，适用于大规模的样品检测，可用于甘蔗育种遗传多样性分析、种质鉴定及指纹图谱构建等。

英文摘要：

This study is an attempt to extract genomie DNA from sugarcane and its related genera including Sacchanun spontaneum, E. arundinaceum and narenga by using the mini-Beadbeater-96 and stainless steel beads. The electrephoresis detection, content determination and SSR analysis were conducted on the DNA obtained from the sugarcane and its related genera. The results showed that this method could extract genomie DNA rapidly and efficiently and the protein and polysaccharose substances in the DNA could be wiped off with better purity and integrality. The strip of SSR analysis was clear with abundant polymorphism,amplifying specific band. This method was simple,rapid, efficient, low cost and good of PCR amplification without grinding in liquid nitrogen. So it was suitable for mass sample test, analyzing genetic diversity of sugarcane, germplasm identification and constructing fingerprint.