中文摘要：

目的：建立得力生注射液中黄芪甲苷的含量测定方法。方法：采用高效液相色谱和蒸发光检测器的方法,Grace Alltima C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈-水（32∶68,v/v）,检测器温度100℃,载气流量为2.7L.min^-1,柱温30℃,流速1 m L.min^-1。进样量为20μL。结果：黄芪甲苷在1.03μg~26.2μg之间呈现良好的线性关系,r=0.9993,平均回收率为100.1%（RSD=3.7%）。结论：建立的得力生注射液中黄芪甲苷含量测定方法简单快速、准确可靠、重复性好,可有效控制得力生注射液质量。

英文摘要：

Objective： To establish a method for the determination of Astragaloside A in Delisheng Injection. Method： The chromatographic conditions of HPLC and the parameters of ELSD were optimized to study the methodology. Analysis was performed on Grace Alltima C18 column（4.6 mm×250 mm, 5 μm） with isocratic elution of acetonitrile–water（32∶68） at the fl ow rate of 1.0 m L.min^-1. Drift tube temperature was maintained at 100℃ and the gas fl ow rate was 2.7 L.min^-1. Result： The calibration curve was linear in the range from 1.03 μg to 26.20 μg（r=0.9993）. The recovery of astragaloside A were 100.1%（RSD=3.7%）. Conclusion： This method is rapid and accurate, and can effectively control the quality of Delisheng Injection.